Nociceptive behavioural assessments in mouse models of temporomandibular joint disorders.
Nociceptive behavioural assessments in mouse models of temporomandibular joint disorders.
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DOI：
10.1038/s41368-020-00095-0
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发表时间：
2020-09-29
影响因子：
14.9
通讯作者：
Chen D
中科院分区：
文献类型：
--
作者：
Li J;Ma K;Yi D;Oh CD;Chen D
口面部疼痛或压痛是与颞下颌关节 (TMJ) 疾病 (TMD) 相关的主要症状。为了了解 TMD 的病理机制，已经开发了几种小鼠模型，包括机械刺激诱导的 TMD 和遗传小鼠模型。然而，缺乏评估小鼠口面部区域TMD相关伤害行为的可行方法，阻碍了TMD相关机制的深入研究。本研究旨在探索对三种现有方法的修改，以使用两种 TMD 小鼠模型来分析伤害性行为：（1）通过将小鼠放置在专门设计的室中，使用小鼠 TMJ 区域的 von Frey 丝测试机械异常性疼痛； (2)使用经济载荷和力(ELF)系统测量咬合力； （3）使用实验动物行为观察登记与分析系统（LABORAS）对自发进食行为测试，包括进食持续时间和频率进行分析。我们成功评估了两种 TMD 小鼠模型的伤害感受行为的变化，即单侧前牙反合 (UAC) 诱导的 TMD 小鼠模型和 β-连环蛋白条件激活小鼠模型。我们发现UAC模型和β-连环蛋白条件激活小鼠模型与机械异常性疼痛增加、咬合力降低和自发进食行为减少的迹象显着相关，表明TMD的表现。这些行为变化与在这些小鼠模型中观察到的软骨退化表型一致。我们的研究显示了分析小鼠伤害性行为的可靠方法，并可能表明这些方法可有效评估小鼠 TMD 迹象。
Orofacial pain or tenderness is a primary symptom associated with temporomandibular joint (TMJ) disorders (TMDs). To understand the pathological mechanisms underlying TMDs, several mouse models have been developed, including mechanical stimulus-induced TMD and genetic mouse models. However, a lack of feasible approaches for assessing TMD-related nociceptive behaviours in the orofacial region of mice has hindered the in-depth study of TMD-associated mechanisms. This study aimed to explore modifications of three existing methods to analyse nociceptive behaviours using two TMD mouse models: (1) mechanical allodynia was tested using von Frey filaments in the mouse TMJ region by placing mice in specially designed chambers; (2) bite force was measured using the Economical Load and Force (ELF) system; and (3) spontaneous feeding behaviour tests, including eating duration and frequency, were analysed using the Laboratory Animal Behaviour Observation Registration and Analysis System (LABORAS). We successfully assessed changes in nociceptive behaviours in two TMD mouse models, a unilateral anterior crossbite (UAC)-induced TMD mouse model and a β-catenin conditional activation mouse model. We found that the UAC model and β-catenin conditional activation mouse model were significantly associated with signs of increased mechanical allodynia, lower bite force, and decreased spontaneous feeding behaviour, indicating manifestations of TMD. These behavioural changes were consistent with the cartilage degradation phenotype observed in these mouse models. Our studies have shown reliable methods to analyse nociceptive behaviours in mice and may indicate that these methods are valid to assess signs of TMD in mice.
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影响因子：
3.6
作者：
Kerins, CA;Carlson, DS;Bellinger, LL
通讯作者：
Bellinger, LL
影响因子：
--
作者：
Aneiros-Guerrero A;Lendinez AM;Palomares AR;Perez-Nevot B;Aguado L;Mayor-Olea A;Ruiz-Galdon M;Reyes-Engel A
通讯作者：
Reyes-Engel A
影响因子：
7.4
作者：
Chen Y;Williams SH;McNulty AL;Hong JH;Lee SH;Rothfusz NE;Parekh PK;Moore C;Gereau RW 4th;Taylor AB;Wang F;Guilak F;Liedtke W
通讯作者：
Liedtke W
影响因子：
2.9
作者：
Kramer, Phillip R.;Kerins, Carolyn A.;Bellinger, Larry L.
通讯作者：
Bellinger, Larry L.
影响因子：
2.4
作者：
ARCAN, M;ZANDMAN, F
通讯作者：
ZANDMAN, F