Homeostatic response to hypoxia is regulated by the N-end rule pathway in plants.
Homeostatic response to hypoxia is regulated by the N-end rule pathway in plants.
复制标题
DOI：
10.1038/nature10534
复制
发表时间：
2011-10-23
期刊：
影响因子：
64.8
通讯作者：
Holdsworth, Michael J.
中科院分区：
文献类型：
--
作者：
Gibbs, Daniel J.;Lee, Seung Cho;Isa, Nurulhikma Md;Gramuglia, Silvia;Fukao, Takeshi;Bassel, George W.;Correia, Cristina Sousa;Corbineau, Francoise;Theodoulou, Frederica L.;Bailey-Serres, Julia;Holdsworth, Michael J.
植物和动物是专性需氧菌，需要氧气进行线粒体呼吸和能量生产。在植物中，由于根渍或叶子淹没引起的氧气可用性（缺氧）的意外下降引发基因转录和mRNA翻译的变化，促进无氧代谢，从而维持底物水平的ATP生产。与动物相比，植物中的氧感受并不归因于对氧剥夺的基因调控机制。在这里，我们表明，N-末端规则的靶向蛋白水解途径作为一个稳态传感器的严重低氧在拟南芥中，通过其调节关键的缺氧反应转录因子。我们发现缺乏N端规则途径成分的植物组成型表达核心缺氧反应基因，并且对缺氧应激更耐受。我们确定了低氧相关的乙烯反应因子（ERF）的拟南芥第七组转录因子作为底物的这一途径。通过N-末端规则途径对这些蛋白质的调节通过N-末端的特征性保守基序发生，该基序以MetCys-（MC-）起始。其中一种蛋白质HRE 2在低氧条件下的稳定性增强，可以改善缺氧生存，并揭示了植物通过进化保守的N端规则途径进行氧传感的分子机制。SUB 1A-1是水稻耐淹性的主要决定因子，尽管含有N-末端基序，但它不是N-末端规则途径的底物，表明它与N-末端规则途径的调节是不偶联的，并且增强的稳定性可能与Sub1水稻品种对多种非生物胁迫的上级耐受性有关。
Plants and animals are obligate aerobes, requiring oxygen for mitochondrial respiration and energy production. In plants, an unanticipated decline in oxygen availability (hypoxia), as caused by root waterlogging or foliage submergence, triggers changes in gene transcription and mRNA translation that promote anaerobic metabolism and thus sustain substrate-level ATP production. In contrast to animals, oxygen sensing has not been ascribed to a mechanism of gene regulation in response to oxygen deprivation in plants. Here we show that the N-end rule pathway of targeted proteolysis acts as a homeostatic sensor of severe low oxygen in Arabidopsis, through its regulation of key hypoxia response transcription factors. We found that plants lacking components of the N-end rule pathway constitutively express core hypoxia response genes and are more tolerant of hypoxic stress. We identify the hypoxia-associated Ethylene Response Factor (ERF) Group VII transcription factors of Arabidopsis as substrates of this pathway. Regulation of these proteins by the N-end rule pathway occurs through a characteristic conserved motif at the N-terminus initiating with MetCys- (MC-). Enhanced stability of one of these proteins, HRE2, under low oxygen conditions improves hypoxia survival and reveals a molecular mechanism for oxygen sensing in plants via the evolutionarily conserved N-end rule pathway. SUB1A-1, a major determinant of submergence tolerance in rice, was shown not to be a substrate for the N-end rule pathway despite containing the N-terminal motif, suggesting that it is uncoupled from N-end rule pathway regulation, and that enhanced stability may relate to the superior tolerance of Sub1 rice varieties to multiple abiotic stresses.
登录
查看更多内容
影响因子：
11.6
作者：
Fukao, Takeshi;Yeung, Elaine;Bailey-Serres, Julia
通讯作者：
Bailey-Serres, Julia
影响因子：
7.4
作者：
Horan, Kevin;Jang, Charles;Girke, Thomas
通讯作者：
Girke, Thomas
影响因子：
56.9
作者：
BACHMAIR, A;FINLEY, D;VARSHAVSKY, A
通讯作者：
VARSHAVSKY, A
影响因子：
7.4
作者：
Hinz, Manuela;Wilson, Iain W.;Dolferus, Rudy
通讯作者：
Dolferus, Rudy
对低氧胁迫转录组调整的跨王国比较凸显了植物的保守反应和特异反应
影响因子：
7.4
作者：
Mustroph, Angelika;Lee, Seung Cho;Bailey-Serres, Julia
通讯作者：
Bailey-Serres, Julia