Impact assessment of cadmium toxicity and its bioavailability in human cell lines (Caco-2 and HL-7702).
Impact assessment of cadmium toxicity and its bioavailability in human cell lines (Caco-2 and HL-7702).
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DOI：
10.1155/2014/839538
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发表时间：
2014
影响因子：
--
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Aziz R;Rafiq MT;Yang J;Liu D;Lu L;He Z;Daud MK;Li T;Yang X
镉（Cd）是一种广泛存在的环境有毒污染物，对人体健康造成严重危害。本研究以人肠细胞系（Caco-2细胞）和正常人肝细胞系（HL-7702细胞）为研究对象，探讨镉对两种细胞系的毒性和生物利用度，并验证这两种细胞系作为研究镉在人肠和肝中蓄积和毒性的体外模型。结果表明，两种细胞系对Cd的摄取均呈剂量依赖性增加，在10 mg L−1时，Caco-2细胞对Cd的摄取（720.15 µg mg−1细胞蛋白）显著高于HL-7702细胞（229.01 µg mg−1细胞蛋白）。一个时间和剂量依赖性的影响，镉对细胞毒性试验（LDH释放，MTT法）中观察到镉处理的细胞系。HL-7702细胞的抗氧化酶活性和分化标志物（SOD、GPX和AKP）均高于Caco-2细胞，但二者均随镉浓度升高而显著降低。本研究结果表明，镉超过一定水平抑制细胞的抗氧化活性，HL-7702细胞比Caco-2细胞更敏感的镉暴露。然而，Cd浓度<0.5 mg L−1对两种细胞系均无毒性作用。
Cadmium (Cd) is a widespread environmental toxic contaminant, which causes serious health-related problems. In this study, human intestinal cell line (Caco-2 cells) and normal human liver cell line (HL-7702 cells) were used to investigate the toxicity and bioavailability of Cd to both cell lines and to validate these cell lines as in vitro models for studying Cd accumulation and toxicity in human intestine and liver. Results showed that Cd uptake by both cell lines increased in a dose-dependent manner and its uptake by Caco-2 cells (720.15 µg mg−1 cell protein) was significantly higher than HL-7702 cells (229.01 µg mg−1 cell protein) at 10 mg L−1. A time- and dose-dependent effect of Cd on cytotoxicity assays (LDH release, MTT assay) was observed in both Cd-treated cell lines. The activities of antioxidant enzymes and differentiation markers (SOD, GPX, and AKP) of the HL-7702 cells were higher than those of Caco-2 cells, although both of them decreased significantly with raising Cd levels. The results from the present study indicate that Cd above a certain level inhibits cellular antioxidant activities and HL-7702 cells are more sensitive to Cd exposure than Caco-2 cells. However, Cd concentrations <0.5 mg L−1 pose no toxic effects on both cell lines.
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DOI：
10.1002/aheh.19910190314
发表时间：
1991-01-01
期刊：
ACTA HYDROCHIMICA ET HYDROBIOLOGICA
影响因子：
--
作者：
LAKSHMI, R;KUNDU, R;MANSURI, AP
通讯作者：
MANSURI, AP
影响因子：
4.5
作者：
Nzengue, Yves;Steiman, Regine;Guiraud, Pascale
通讯作者：
Guiraud, Pascale
影响因子：
3.3
作者：
Acharya, Usha R.;Mishra, Monalisa;Panda, Manoj K.
通讯作者：
Panda, Manoj K.
影响因子：
2.8
作者：
Elsenhans, B;Strugala, GJ;Schafer, SG
通讯作者：
Schafer, SG
镉诱导的两个转基因棉花品种及其野生近缘种叶片的超形态和生理变化
影响因子：
13.6
作者：
Daud, M. K.;Variath, M. T.;Zhu, Shuijin
通讯作者：
Zhu, Shuijin