Mapping and introgression of QTL involved in fruit shape transgressive segregation into ‘piel de sapo’ melon (cucumis melo l.) [corrected].
Mapping and introgression of QTL involved in fruit shape transgressive segregation into ‘piel de sapo’ melon (cucumis melo l.) [corrected].
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DOI：
10.1371/journal.pone.0104188
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Monforte AJ
中科院分区：
文献类型：
--
作者：
Díaz A;Zarouri B;Fergany M;Eduardo I;Alvarez JM;Picó B;Monforte AJ
对‘Piel de Sapo’×PI124112杂交的F2群体进行选择性基因分型，通过QTL（数量性状基因座）分析研究果实形态性状的遗传控制。确定了 10 个 QTL，其中 5 个用于 FL（果实长度），2 个用于 FD（果实直径），3 个用于 FS（果实形状）。每个字符至少发现一个稳健的 QTL，flqs8.1 (LOD = 16.85，R2 = 34%)、fdqs12.1 (LOD = 3.47，R2 = 11%) 和 fsqs8.1 (LOD = 14.85， R2 = 41%）。 flqs2.1 和 fsqs2.1 与基因 a（雄性同花）共分离，负责花性别决定并对 FS 具有多效性作用。它们表现出正加性效应 (a) 值，因此 PI124112 等位基因会导致 FL 和 FS 增加，从而产生更细长的果实。相反，flqs8.1 和 fsqs8.1 的负 a 值表示 FL 和 FS 降低，这会导致果实更圆，即使 PI124112 生产出非常细长的瓜。这是通过 fsqs2.1 和 fsqs8.1 之间显着的上位相互作用来解释的，其中基因座 a 上的等位基因的影响被 fsqs8.1 上的附加 PI124112 等位基因减弱。最圆的果实是由 fsqs8.1 上的 PI124112 纯合子产生的，其在基因座 a (PiPiaa) 处不携带任何显性 A 等位基因。还检测到 fsqs8.1 和 fsqs12.1 之间存在显着的相互作用，fsqs12.1 的等位基因产生更细长的果实。 fsqs8.1 似乎与在其他种群中发现的 QTL 等位基因，在这些种群中，外来等位基因产生细长的果实。该模型已在长达 3 年的回交系测定中得到验证，最终在“Piel de Sapo”背景中获得了 fsqs8.1-NIL（近等基因系），可产出圆形甜瓜。
A mapping F2 population from the cross ‘Piel de Sapo’ × PI124112 was selectively genotyped to study the genetic control of morphological fruit traits by QTL (Quantitative Trait Loci) analysis. Ten QTL were identified, five for FL (Fruit Length), two for FD (Fruit Diameter) and three for FS (Fruit Shape). At least one robust QTL per character was found, flqs8.1 (LOD = 16.85, R2 = 34%), fdqs12.1 (LOD = 3.47, R2 = 11%) and fsqs8.1 (LOD = 14.85, R2 = 41%). flqs2.1 and fsqs2.1 cosegregate with gene a (andromonoecious), responsible for flower sex determination and with pleiotropic effects on FS. They display a positive additive effect (a) value, so the PI124112 allele causes an increase in FL and FS, producing more elongated fruits. Conversely, the negative a value for flqs8.1 and fsqs8.1 indicates a decrease in FL and FS, what results in rounder fruits, even if PI124112 produces very elongated melons. This is explained by a significant epistatic interaction between fsqs2.1 and fsqs8.1, where the effects of the alleles at locus a are attenuated by the additive PI124112 allele at fsqs8.1. Roundest fruits are produced by homozygous for PI124112 at fsqs8.1 that do not carry any dominant A allele at locus a (PiPiaa). A significant interaction between fsqs8.1 and fsqs12.1 was also detected, with the alleles at fsqs12.1 producing more elongated fruits. fsqs8.1 seems to be allelic to QTL discovered in other populations where the exotic alleles produce elongated fruits. This model has been validated in assays with backcross lines along 3 years and ultimately obtaining a fsqs8.1-NIL (Near Isogenic Line) in ‘Piel de Sapo’ background which yields round melons.
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影响因子：
56.9
作者：
Boualem, Adnane;Fergany, Mohamed;Bendahmane, Abdelhafid
通讯作者：
Bendahmane, Abdelhafid
影响因子：
64.8
作者：
Doebley, J;Stec, A;Hubbard, L
通讯作者：
Hubbard, L
影响因子：
30.8
作者：
Cong, Bin;Barrero, Luz S.;Tanksley, Steven D.
通讯作者：
Tanksley, Steven D.
影响因子：
5.4
作者：
Eduardo, I;Arús, P;Monforte, AJ
通讯作者：
Monforte, AJ
影响因子：
3.3
作者：
Chen, KY;Tanksley, SD
通讯作者：
Tanksley, SD