Curcumin inhibited hepatitis B viral entry through NTCP binding.
Curcumin inhibited hepatitis B viral entry through NTCP binding.
复制标题
姜黄素通过与NtCP结合抑制乙肝病毒侵入。
DOI：
10.1038/s41598-021-98243-x
复制
发表时间：
2021-09-27
影响因子：
4.6
通讯作者：
Sa-Ngiamsuntorn K
中科院分区：
文献类型：
--
作者：
Thongsri P;Pewkliang Y;Borwornpinyo S;Wongkajornsilp A;Hongeng S;Sa-Ngiamsuntorn K
B型肝炎病毒（HBV）与肝炎和肝细胞癌有关。目前的药物（核苷（酸）类似物和干扰素）只能减弱HBV感染。靶向病毒生命周期不同阶段的药剂组合（例如，进入、复制和cccDNA稳定性）预期能够根除感染。研究姜黄素（CCM）对HBV附着和内化的抑制作用。永生化肝细胞样细胞（imHC）、HepaRG和非肝细胞作为宿主细胞，用于与CCM的结合研究。CCM降低病毒载量、HBeAg、HBcAg（感染性）、细胞内HBV DNA和cccDNA水平。CCM诱导的HBV进入抑制与牛磺胆酸钠共转运多肽（NTCP）的密度直接相关，NTCP是HBV进入的已知宿主受体。CCM的作用部位用TCA摄取试验证实。使用等温滴定量热法（ITC）测量CCM与NTCP之间的亲和力。这些结果表明，CCM中断HBV进入，因此将抑制HBV再感染。
Hepatitis B virus (HBV) has been implicated in hepatitis and hepatocellular carcinoma. Current agents (nucleos(t)ide analogs and interferons) could only attenuate HBV infection. A combination of agents targeting different stages of viral life cycle (e.g., entry, replication, and cccDNA stability) was expected to eradicate the infection. Curcumin (CCM) was investigated for inhibitory action toward HBV attachment and internalization. Immortalized hepatocyte-like cells (imHCs), HepaRG and non-hepatic cells served as host cells for binding study with CCM. CCM decreased viral load, HBeAg, HBcAg (infectivity), intracellular HBV DNA, and cccDNA levels. The CCM-induced suppression of HBV entry was directly correlated with the density of sodium-taurocholate co-transporting polypeptide (NTCP), a known host receptor for HBV entry. The site of action of CCM was confirmed using TCA uptake assay. The affinity between CCM and NTCP was measured using isothermal titration calorimetry (ITC). These results demonstrated that CCM interrupted HBV entry and would therefore suppress HBV re-infection.
登录
查看更多内容
DOI：
10.1002/hep.29323
发表时间：
2017-10
期刊：
Hepatology (Baltimore, Md.)
影响因子：
--
作者：
Lok AS;Zoulim F;Dusheiko G;Ghany MG
通讯作者：
Ghany MG
影响因子：
3.7
作者：
Chen TY;Chen DY;Wen HW;Ou JL;Chiou SS;Chen JM;Wong ML;Hsu WL
通讯作者：
Hsu WL
影响因子：
3.4
作者：
Herrscher, Charline;Pastor, Florentin;Blanchard, Emmanuelle
通讯作者：
Blanchard, Emmanuelle
影响因子：
--
作者：
Moghadamtousi SZ;Kadir HA;Hassandarvish P;Tajik H;Abubakar S;Zandi K
通讯作者：
Zandi K
影响因子：
25.7
作者：
Konig, Alexander;Yang, Jaewon;Windisch, Marc Peter
通讯作者：
Windisch, Marc Peter