Circulating endothelial progenitor cells inversely associate with organ dysfunction in sepsis.
Circulating endothelial progenitor cells inversely associate with organ dysfunction in sepsis.
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DOI：
10.1007/s00134-012-2480-9
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发表时间：
2012-03
影响因子：
38.9
通讯作者：
Martin, Greg S.
中科院分区：
文献类型：
--
作者：
Cribbs, Sushma K.;Sutcliffe, Diane J.;Taylor, William R.;Rojas, Mauricio;Easley, Kirk A.;Tang, Li;Brigham, Kenneth L.;Martin, Greg S.
内皮功能障碍是脓毒症相关器官功能障碍和死亡的主要原因。在脓毒症动物模型中，内皮祖细胞（EPC）有助于血管修复。内皮祖细胞作为器官功能障碍的生物标志物的作用仍然是未知的。我们假设循环中内皮祖细胞的数量与脓毒症预后的改善有关。2007年7月至2009年4月在埃默里大学附属格雷迪纪念医院成人重症监护病房进行的前瞻性、观察性单中心队列研究。从95名脓毒症患者、37名重症监护室对照和51名健康对照中获得外周血，其中仅86名脓毒症患者用于分析，因为我们无法在9名脓毒症患者中获得足够的血液。获得临床数据，并通过脓毒症相关器官衰竭评估（SOFA）评分测量器官功能障碍。通过集落形成单位（CFU）测定评估内皮祖细胞，其中使用Ficoll密度梯度离心分离外周血单核细胞并在生长培养基中培养。与重症监护室对照组（p = 0.035）和健康对照组（p = 0.0005）相比，脓毒症患者的平均内皮祖细胞集落计数显著降低。ICU对照组和健康对照组之间的菌落计数无差异（p = 0.81）。在脓毒症患者中，EPC CFU数量与SOFA评分呈负相关，校正死亡率（r2 = 0.05，p = 0.04）。败血症患者循环内皮祖细胞增加与器官功能障碍呈负相关。
Endothelial dysfunction is a primary contributor to sepsis-related organ dysfunction and death. In sepsis animal models, endothelial progenitor cells (EPC) have contributed to vascular repair. The role of endothelial progenitor cells as a biomarker for organ dysfunction is still unknown. We hypothesized that circulating numbers of endothelial progenitor cells would be associated with improved outcomes in sepsis. Prospective, observational single-center cohort study in adult intensive care units at Grady Memorial Hospital, an affiliate of Emory University, from July 2007 through April 2009. Peripheral blood was obtained from 95 patients with sepsis, 37 intensive care unit controls, and 51 healthy controls, of whom only 86 patients with sepsis were used in the analysis because we were not able to obtain enough blood in 9 sepsis patients. Clinical data were obtained, and organ dysfunction was measured by Sepsis-Related Organ Failure Assessment (SOFA) score. Endothelial progenitor cells were assessed by a colony-forming unit (CFU) assay in which peripheral blood mononuclear cells were isolated using Ficoll density-gradient centrifugation and cultured in growth media. The patients with sepsis had significantly lower mean endothelial progenitor cell colony counts compared with intensive care unit controls (p = 0.035) and healthy controls (p = 0.0005). There was no difference in colony counts between ICU controls and healthy controls (p = 0.81). In the sepsis patients, EPC CFU numbers inversely associated with SOFA score, adjusting for mortality (r2 = 0.05, p = 0.04). Increased circulating endothelial progenitor cells inversely correlate with organ dysfunction in sepsis patients.
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影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
影响因子：
4.4
作者：
Gupta, Naveen;Su, Xiao;Matthay, Michael A.
通讯作者：
Matthay, Michael A.
DOI：
10.1165/rcmb.2009-0015oc
发表时间：
2010-09-01
影响因子：
6.4
作者：
Burnham, Ellen L.;Mealer, Meredith;Moss, Marc
通讯作者：
Moss, Marc
影响因子：
20.1
作者：
Assmus, Birgit;Fischer-Rasokat, Ulrich;Zeiher, Andreas M.
通讯作者：
Zeiher, Andreas M.
DOI：
10.1177/039463200802100324
发表时间：
2008-07-01
影响因子：
3.5
作者：
Becchi, C.;Pillozzi, S.;Amedei, A.
通讯作者：
Amedei, A.