Endogenous Retrovirus ev21 Dose Not Recombine with ALV-J and Induces the Expression of ISGs in the Host.
Endogenous Retrovirus ev21 Dose Not Recombine with ALV-J and Induces the Expression of ISGs in the Host.
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内源性逆转录病毒ev21不与ALV-J重组并诱导宿主中ISG的表达
DOI：
10.3389/fcimb.2016.00140
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发表时间：
2016
影响因子：
5.7
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Feng M;Tan Y;Dai M;Li Y;Xie T;Li H;Shi M;Zhang X
禽白血病病毒J亚群（ALV-J）感染可引起肿瘤和免疫抑制。内源性病毒整合到宿主基因组中，并能与外源性禽白血病病毒（ALV）重组。在本研究中，我们分析了内源性逆转录病毒21 （ev21）与晚羽化中国黄鸡ALV-J的相互作用。从中国黄鸡群中分离到2株ALV-J菌株M180和K243。两株毒株的env基因与ALV-J参考株的核苷酸同源性为94.2 ~ 94.8%。与ev21和M180的env基因和LTR相比，LTR的核苷酸同源性为69.7%，env基因的核苷酸同源性为58.4%，尤其是env基因的氨基酸同源性低至14.2%。对env基因和3′ltr核苷酸序列的系统发育分析表明，M180与ALV-J亲缘关系密切，在系统发育树上与ev21处于不同的类群。使用共免疫沉淀（co-IP），我们接下来证明ev21的包膜蛋白不与M180包膜蛋白相互作用。通过荧光素酶报告基因检测，我们进一步发现ev21的包膜蛋白不能激活ALV-J LTR启动子的活性。qPCR和western blot分析显示，内源性ev21包膜蛋白在ALV-J感染后6h （hpi）促进PKR的表达，在24 hpi时促进ISG12和CH25H的表达。而M180菌株在6和24 hpi时，env基因的表达不显著。结果表明，内源性逆转录病毒ev21与晚羽化黄鸡ALV-J株M180之间没有重组的证据，ev21的包膜蛋白可以影响宿主ISGs的表达，但似乎不影响ALV-J株M180的复制。这是首次报道内源性逆转录病毒ev21、ALV-J与晚羽化鸡相互作用。
Avian leukosis virus subgroup J (ALV-J) infection can cause tumors and immunosuppression. Endogenous viruses integrate into host genomes and can recombine with exogenous avian leukosis virus (ALV). In this study, we analyzed the interaction of endogenous retrovirus 21 (ev21) with the ALV-J in late-feathering Chinese yellow chicken. Two ALV-J strains M180 and K243 were isolated from late-feathering and fast-feathering Chinese yellow chicken flocks, respectively. The env gene of the two strains showed 94.2–94.8% nucleotide identity with reference ALV-J strains. Compared with the env gene and the LTR of ev21 and M180, the nucleotide identity of LTR was 69.7% and env gene was 58.4%, respectively, especially the amino acid identity of env gene as low as 14.2%. Phylogenetic analysis of the nucleotide sequence of the env gene and the 3′LTR showed that M180 was closely related to ALV-J, and was located in a distinct group with ev21 in the phylogenetic tree. Using co-immunoprecipitation (co-IP), we next demonstrate that the envelope protein of ev21 does not interact with the M180 envelope protein. We further show that the envelope protein of ev21 cannot activate ALV-J LTR promoter activity using luciferase-reporter assays. qPCR and western blot analysis revealed that envelope protein of endogenous ev21 can facilitate the expression of PKR at 6h post ALV-J infection (hpi) and facilitate the expression of ISG12 and CH25H at 24 hpi. However, the expression of the env gene of M180 strain was not significantly at 6 and 24 hpi. We conclude that there is no evidence of recombination between endogenous retrovirus ev21 and ALV-J strain M180 in late-feathering Chinese yellow chicken, and envelope protein of ev21 can affect the expression of host ISGs, but appears not to influence the replication of ALV-J strain M180. This is the first report of interaction among the endogenous retrovirus ev21, ALV-J and the late-feathering chicken.
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影响因子：
5.9
作者：
Schoggins, John W.;Rice, Charles M.
通讯作者：
Rice, Charles M.
影响因子：
3.3
作者：
Lai, Hanzhang;Zhang, Henan;Liao, Ming
通讯作者：
Liao, Ming
影响因子：
3.8
作者：
Gao, Yanni;Guan, Xiaolu;Gao, Yulong
通讯作者：
Gao, Yulong
影响因子：
4.8
作者：
Dai M;Feng M;Liu D;Cao W;Liao M
通讯作者：
Liao M
DOI：
10.1126/science.aad5497
发表时间：
2016-03-04
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chuong EB;Elde NC;Feschotte C
通讯作者：
Feschotte C