Expanding dynamics of the virulence-related gene variations in the toxigenic Vibrio cholerae serogroup O1
Expanding dynamics of the virulence-related gene variations in the toxigenic Vibrio cholerae serogroup O1
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产毒霍乱弧菌 O1 群毒力相关基因变异的动态扩展
DOI：
10.1186/s12864-019-5725-y
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发表时间：
2019-05
期刊：
影响因子：
4.4
通讯作者：
Kan Biao
中科院分区：
文献类型：
--
作者：
Li Zhenpeng;Pang Bo;Wang Duochun;Li Jie;Xu Jialiang;Fang Yujie;Lu Xin;Kan Biao
产毒霍乱弧菌血清群O1是第六次和第七次霍乱大流行的致病病原体。霍乱毒素是主要的毒力因子，但其他毒力和毒力相关因子在宿主的发病和生存中也起着一定的作用。随着流行毒株的进化，毒力相关基因也会发生变异和增减，导致不同毒株之间出现遗传分化。在这项研究中，我们分析了1923年至2015年分离的产毒血清群O1菌株的毒力相关基因谱，这些菌株的基因组是公开的。根据毒力因子数据库（VFDB）对霍乱弧菌O1株的毒力相关基因进行了注释。每株平均鉴定出230.1个毒力相关基因；经典型和El Tor生物型菌株的平均毒力相关基因数存在显著差异，El Tor生物型菌株的毒力相关基因数随分离年限的增加呈增加趋势。共检出176个同源毒力相关基因，其中25个属于核心基因，提示其在霍乱弧菌发病过程中起着保守而必要的作用。我们通过定义基因序列类型来描述同系物的多样性，并说明其与基因重复的关系；我们发现基因重复明显增加了核心毒力相关基因序列类型的复杂性。此外，我们还从基因的得失、变异、基因重复和基因序列型数等方面提供了毒力相关基因的遗传特征与分离年限有关的基因图谱。本研究揭示了产毒霍乱弧菌O1血清群毒力相关基因在流行期间的综合变异动态。毒力相关基因的增加趋势可能表明菌株通过获得不同功能类别的毒力相关基因而具有进化优势。
Toxigenic Vibrio cholerae serogroup O1 is the causative pathogen in the sixth and seventh cholera pandemics. Cholera toxin is the major virulent factor but other virulence and virulence-related factors play certain roles in the pathogenesis and survival in the host. Along with the evolution of the epidemic strains, the virulence-related genes also experience variation, gain and loss, and lead to genetic divergence in different strains. In this study, we analyzed the virulence-related gene profiles in the toxigenic serogroup O1 strains isolated from 1923 to 2015, the genomes of which were publicly available. The virulence-related genes of the V. cholerae O1 strains were annotated based on the Virulence Factors Database (VFDB). An average of 230.1 virulence-related genes per strain were identified; significant differences in the average numbers were found between the classical and El Tor biotypes, and increasing trends in the number of virulence-related genes along with the isolation years were observed in the El Tor biotype strains. A total of 176 homologs of virulence-related genes were found from these strains, of which 25 belonged to the core genes, suggesting their conservative and necessary roles in V. cholerae pathogenesis. We described the diversities of the homologs by defining gene sequence type, and illustrated its association with gene duplication; we found that gene duplication clearly increased the complexity of the gene sequence types in the core virulence-related genes. In addition, we provided virulence-related gene profiles whose genetic characteristic depend on the isolation years from the view of gene gain and loss, variation, gene duplication and gene sequence type number. Our study reveals the comprehensive variation dynamics of the virulence-related genes in toxigenic V. cholerae serogroup O1 during epidemics. The increasing trend for the virulence-related genes may suggest the evolutional advantage of strains by gaining virulence-related genes with diverse functional categories.
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影响因子：
3.7
作者：
Lippi, Donatella;Gotuzzo, Eduardo;Caini, Saverio
通讯作者：
Caini, Saverio
DOI：
10.1093/bioinformatics/bts565
发表时间：
2012-12-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Fu L;Niu B;Zhu Z;Wu S;Li W
通讯作者：
Li W
影响因子：
56.9
作者：
Weill, Francois-Xavier;Domman, Daryl;Thomson, Nicholas R.
通讯作者：
Thomson, Nicholas R.
影响因子：
168.9
作者：
Harris, Jason B.;LaRocque, Regina C.;Calderwood, Stephen B.
通讯作者：
Calderwood, Stephen B.
DOI：
10.1136/bmj.2.1863.683
发表时间：
1885-09
期刊：
Atlanta Medical and Surgical Journal (1884)
影响因子：
--
作者：
A. Afkhami
通讯作者：
A. Afkhami