Chemiluminescent immunoassay of thyroxine enhanced by microchip electrophoresis.
Chemiluminescent immunoassay of thyroxine enhanced by microchip electrophoresis.
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DOI：
10.1016/j.ab.2009.11.036
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发表时间：
2010-04-01
影响因子：
2.9
通讯作者：
Liu YM
中科院分区：
文献类型：
--
作者：
Huang Y;Zhao S;Shi M;Liu YM
建立了微芯片电泳法强化甲状腺激素(T4)的均相化学发光免疫分析方法。该方法利用T4和辣根过氧化物酶(HRP)标记的T4(HRP-T4)与抗T4鼠单抗竞争免疫反应。采用微芯片电泳法(MCE)和化学发光(CL)检测法对HRP-T4和HRP-T4-Ab复合体进行分离和定量。通过HPR催化的鲁米诺-H_2O_2反应，实现了高灵敏度的化学发光检测。由于有效的MCE分离，化学发光分析信号不容易受到样品基质的干扰。在选定的分析条件下，MCE分离在60秒内完成。T4的线性范围为5-250 nm，检出限为2.2 nm(S/N=3)。该方法已成功应用于人血清中T4的测定。结果表明，本方法具有快速、灵敏、选择性高的特点。它可作为临床分析T4的工具，辅助甲状腺功能的诊断。
A homogenous chemiluminescent immunoassay of thyroxine (T4) enhanced by microchip electrophoresis separation has been developed. The method deployed the competitive immunoreaction of T4 and horseradish peroxidase (HRP)-labeled T4 (HRP–T4) with anti-T4 mouse monoclonal antibody (Ab). HRP–T4 and the HRP–T4–Ab complex were separated and quantified by using microchip electrophoresis (MCE) with chemiluminescence (CL) detection. Highly sensitive CL detection was achieved by means of HPR-catalyzed luminol-H2O2 reaction. Due to the effective MCE separation, the CL analytical signal was less prone to sample matrix interference. Under the selected assay conditions, the MCE separation was accomplished within 60 sec. The linear range for T4 were 5-250 nM with a detection limit of 2.2 nM (S/N = 3). The present method was successfully applied for the quantification of T4 in human serum samples. It was demonstrated that the present MCE-CL enhanced competitive immunoassay was quick, sensitive, and highly selective. It may serve as a tool for clinical analysis of T4 to assist diagnosis of thyroid gland functions.
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影响因子：
4.1
作者：
Wang, Ai-Jun;Feng, Jiu-Ju;Fan, Jing
通讯作者：
Fan, Jing
影响因子：
5
作者：
GONZALEZ, RR;ROBAINA, R;BLANCA, S
通讯作者：
BLANCA, S
影响因子：
7.4
作者：
Koutny, LB;Schmalzing, D;Fuchs, M
通讯作者：
Fuchs, M
影响因子：
2.9
作者：
Armenta, Jenny M.;Dawoud, Abdulilah A.;Lazar, Iulia M.
通讯作者：
Lazar, Iulia M.
影响因子：
2.9
作者：
He, ZH;Jin, WR
通讯作者：
Jin, WR