Bases in 16S rRNA important for subunit association, tRNA binding, and translocation.
Bases in 16S rRNA important for subunit association, tRNA binding, and translocation.
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DOI：
10.1021/bi900472a
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发表时间：
2009-07-28
期刊：
影响因子：
2.9
通讯作者：
Joseph, Simpson
中科院分区：
文献类型：
--
作者：
Shi, Xinying;Chiu, Katie;Ghosh, Srikanta;Joseph, Simpson
Ribosomes are the cellular machinery responsible for protein synthesis. A well-orchestrated step in the elongation cycle of protein synthesis is the precise translocation of the tRNA-mRNA complex within the ribosome. Here we report the application of a new in vitro modification-interference method for the identification of bases in 16S rRNA that are essential for translocation. Our results suggest that conserved bases U56, U723, A1306, A1319, and A1468 in 16S rRNA are important for translocation. These five bases were deleted or mutated in order to study their role in translation. Depending on the type of mutation, we observed inhibition of growth rate, subunit association, tRNA binding and/or translocation. Interestingly, deletion of U56 or A1319 or mutation of A1319 to C showed a lethal phenotype and were defective in protein synthesis in vitro. Further analysis showed that deletion of U56 or A1319 caused defects in 30S subunit assembly, subunit association and tRNA binding. In contrast, A1319C mutation showed no defects in subunit association; however, the extent of tRNA binding and translocation was significantly reduced. These results show that conserved bases located as far away as 100 Å from the tRNA binding sites can be important for translation.
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DOI：
10.1038/10695
发表时间：
1999-07-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Agrawal, RK;Heagle, AB;Frank, J
通讯作者：
Frank, J
DOI：
10.1073/pnas.211184098
发表时间：
2001-09-25
影响因子：
11.1
作者：
Feinberg, JS;Joseph, S
通讯作者：
Joseph, S
DOI：
10.1073/pnas.96.5.1971
发表时间：
1999-03-02
影响因子：
11.1
作者：
Asai, T;Zaporojets, D;Squires, CL
通讯作者：
Squires, CL
影响因子：
64.5
作者：
Brodersen, DE;Clemons, WM;Ramakrishnan, V
通讯作者：
Ramakrishnan, V
影响因子：
5.7
作者：
Kaminishi, Tatsuya;Wilson, Daniel N.;Yokoyama, Shigeyuki
通讯作者：
Yokoyama, Shigeyuki