BAK1 Mediates Light Intensity to Phosphorylate and Activate Catalases to Regulate Plant Growth and Development
BAK1 Mediates Light Intensity to Phosphorylate and Activate Catalases to Regulate Plant Growth and Development
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BAK1 介导光强度磷酸化和激活过氧化氢酶以调节植物生长和发育
DOI：
10.3390/ijms21041437
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发表时间：
2020-02
影响因子：
5.6
通讯作者：
Xiaofeng Wang
中科院分区：
文献类型：
--
作者：
Shan Zhang;Cheng Li;Haihua Ren;Tong Zhao;Qi Li;Shufen Wang;Yanfeng Zhang;Fangming Xiao;Xiaofeng Wang
BAK 1（brassinosteroid-insensitive 1，BRI 1）associated receptor kinase 1）作为一种辅助受体，在多种信号转导途径中发挥重要作用，调控植物的生长发育和逆境应答。然而，BAK 1在强光信号传导中的作用仍然知之甚少。我们观察到BAK 1在拟南芥中的过表达干扰了强光促进植物生长发育的功能，这是不依赖于油菜素类固醇（BR）信号通路的。进一步的研究表明，强光增强了BAK 1的磷酸化和过氧化氢酶活性，从而减少了过氧化氢（H2 O2）的积累。过氧化氢酶3（CAT 3）通过亲和纯化和LC-MS/MS分析被鉴定为BAK 1相互作用蛋白。生化分析证实BAK 1与拟南芥基因组的所有三种过氧化氢酶（CAT 1，CAT 2和CAT 3）相互作用并磷酸化，并且通过LC-MS/MS鉴定了三种过氧化氢酶与BAK 1-CD的体外转磷酸化位点。遗传分析表明，BAK 1过表达的植物敲除了所有三个CAT基因，完全消除了BAK 1对强光促进生长的抑制作用。本研究首次揭示了BAK 1在拟南芥中介导高光触发的CAT激活，从而降解H2 O2和调节植物生长发育中的作用。
BAK1 (brassinosteroid-insensitive 1 (BRI1) associated receptor kinase 1) plays major roles in multiple signaling pathways as a coreceptor to regulate plant growth and development and stress response. However, the role of BAK1 in high light signaling is still poorly understood. Here we observed that overexpression of BAK1 in Arabidopsis interferes with the function of high light in promoting plant growth and development, which is independent of the brassinosteroid (BR) signaling pathway. Further investigation shows that high light enhances the phosphorylation of BAK1 and catalase activity, thereby reducing hydrogen peroxide (H2O2) accumulation. Catalase3 (CAT3) is identified as a BAK1-interacting protein by affinity purification and LC-MS/MS analysis. Biochemical analysis confirms that BAK1 interacts with and phosphorylates all three catalases (CAT1, CAT2, and CAT3) of the Arabidopsis genome, and the trans-phosphorylation sites of three catalases with BAK1-CD are identified by LC-MS/MS in vitro. Genetic analyses reveal that the BAK1 overexpression plants knocked out all the three CAT genes completely abolishing the effect of BAK1 on suppression of high light-promoted growth. This study first unravels the role of BAK1 in mediating high light-triggered activation of CATs, thereby degrading H2O2 and regulating plant growth and development in Arabidopsis.
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影响因子：
6.9
作者：
Zhou J;Wang J;Li X;Xia XJ;Zhou YH;Shi K;Chen Z;Yu JQ
通讯作者：
Yu JQ
影响因子：
4.9
作者：
Betsuyaku S;Takahashi F;Kinoshita A;Miwa H;Shinozaki K;Fukuda H;Sawa S
通讯作者：
Sawa S
影响因子：
64.5
作者：
Li, J;Wen, JQ;Walker, JC
通讯作者：
Walker, JC
DOI：
10.1101/pdb.prot4995
发表时间：
2008-04-01
期刊：
CSH protocols
影响因子：
--
作者：
Waadt, Rainer;Kudla, Jorg
通讯作者：
Kudla, Jorg
影响因子：
11.6
作者：
Zhou, Yan-Biao;Liu, Cong;Liu, Xuan-Ming
通讯作者：
Liu, Xuan-Ming