Mitogen-activated protein kinase regulated by the CLAVATA receptors contributes to shoot apical meristem homeostasis.
Mitogen-activated protein kinase regulated by the CLAVATA receptors contributes to shoot apical meristem homeostasis.
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DOI：
10.1093/pcp/pcq157
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发表时间：
2011-01
影响因子：
4.9
通讯作者：
Sawa S
中科院分区：
文献类型：
--
作者：
Betsuyaku S;Takahashi F;Kinoshita A;Miwa H;Shinozaki K;Fukuda H;Sawa S
在拟南芥中，CLAVATA （CLV）通路参与调控茎尖分生组织（SAM）中干细胞群体的大小。CLV3作为一种小肽配体，通过三种主要受体激酶复合物CLV1、CLV2-SUPPRESSOR of LLP1-2 (SOL2)/CORYNE （CRN）和最近发现的受体样蛋白激酶2 (RPK2)/毒菌2 （TOAD2）负性调节WUSCHEL （WUS）转录因子的表达。为了了解CLV3信号传导的精确分子细节，我们研究了可能由这些激酶复合物调节的磷酸化信号传导对CLV通路的贡献。我们检测到clv3触发的CLV1磷酸化，这也受到其他CLV受体的制约，可能是通过它们的直接关联。我们对不同CLV受体对丝裂原活化蛋白激酶（MAPK）活性的综合分析表明，CLV受体对MAPK活性的精确平衡调节可能是SAM稳态的关键。
In Arabidopsis, the CLAVATA (CLV) pathway operates in the regulation of the size of the stem cell population in the shoot apical meristem (SAM). CLV3 functions as a small peptide ligand to negatively regulate the expression of the WUSCHEL (WUS) transcription factor through three major receptor kinase complexes of CLV1, CLV2-SUPPRESSOR OF LLP1-2 (SOL2)/CORYNE (CRN) and recently identified RECEPTOR-LIKE PROTEIN KINASE 2 (RPK2)/TOADSTOOL 2 (TOAD2). Aiming to understand the precise molecular details of CLV3 signaling, we investigated the contribution of phospho-signaling, potentially regulated by these kinase complexes, to the CLV pathway. We detected CLV3-triggered CLV1 phosphorylation, which is also conditioned by the rest of the CLV receptors, presumably by their direct association. Our comprehensive analysis of the activities of the respective CLV receptors on mitogen-activated protein kinases (MAPKs) suggested that the precise balanced regulation of MAPK activity by the CLV receptors is likely to be key for SAM homeostasis.
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