Neorickettsia risticii surface-exposed proteins: proteomics identification, recognition by naturally-infected horses, and strain variations.
Neorickettsia risticii surface-exposed proteins: proteomics identification, recognition by naturally-infected horses, and strain variations.
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DOI：
10.1186/1297-9716-42-71
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发表时间：
2011-06-02
影响因子：
4.4
通讯作者：
Rikihisa Y
中科院分区：
文献类型：
--
作者：
Gibson KE;Pastenkos G;Moesta S;Rikihisa Y
立氏新立克次体是革兰氏阴性、专性细胞内细菌病原体，可引发波托马克马热（PHF），这是一种马的重要急性全身性疾病。立氏新立克次体的表面蛋白对免疫识别至关重要，但尚未得到充分的特性研究。在本文中，我们通过对链霉亲和素纯化的生物素化表面标记蛋白进行质谱分析，确定51 - kDa抗原（P51）是立氏新立克次体的古老菌株和当代菌株的一种主要的表面暴露外膜蛋白。对自然感染马匹的血清进行的蛋白质印迹分析表明，与其他新立克次体重组蛋白相比，重组P51能被普遍且强烈地识别。对美国各地26年间患有波托马克马热的马匹的立氏新立克次体菌株中P51以及新立克次体表面蛋白2（Nsp2）和3（Nsp3）的预测二级结构的氨基酸序列进行比较后发现，菌株之间的大部分变异集中在预测为其β - 桶状结构的外环区域。在Ssa3的一个串联重复区域内出现了大片段的插入或缺失。这些数据表明了P51的地理关联模式以及Nsp2、Nsp3和Ssa3的时间关联模式，显示了这些新立克次体表面抗原基因的进化趋势。这项研究展示了立氏新立克次体表面蛋白的群体动态，为设计波托马克马热的免疫诊断靶点提供了基础。
Neorickettsia risticii is the Gram-negative, obligate, and intracellular bacterial pathogen responsible for Potomac horse fever (PHF): an important acute systemic disease of horses. N. risticii surface proteins, critical for immune recognition, have not been thoroughly characterized. In this paper, we identified the 51-kDa antigen (P51) as a major surface-exposed outer membrane protein of older and contemporary strains of N. risticii through mass spectrometry of streptavidin-purified biotinylated surface-labeled proteins. Western blot analysis of sera from naturally-infected horses demonstrated universal and strong recognition of recombinant P51 over other Neorickettsia recombinant proteins. Comparisons of amino acid sequences for predicted secondary structures of P51, as well as Neorickettsia surface proteins 2 (Nsp2) and 3 (Nsp3) among N. risticii strains from horses with PHF during a 26-year period throughout the United States revealed that the majority of variations among strains were concentrated in regions predicted to be external loops of their β-barrel structures. Large insertions or deletions occurred within a tandem-repeat region in Ssa3. These data demonstrate patterns of geographical association for P51 and temporal associations for Nsp2, Nsp3, and Ssa3, indicating evolutionary trends for these Neorickettsia surface antigen genes. This study showed N. risticii surface protein population dynamics, providing groundwork for designing immunodiagnostic targets for PHF.
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影响因子：
4.5
作者：
Dunning Hotopp JC;Lin M;Madupu R;Crabtree J;Angiuoli SV;Eisen JA;Seshadri R;Ren Q;Wu M;Utterback TR;Smith S;Lewis M;Khouri H;Zhang C;Niu H;Lin Q;Ohashi N;Zhi N;Nelson W;Brinkac LM;Dodson RJ;Rosovitz MJ;Sundaram J;Daugherty SC;Davidsen T;Durkin AS;Gwinn M;Haft DH;Selengut JD;Sullivan SA;Zafar N;Zhou L;Benahmed F;Forberger H;Halpin R;Mulligan S;Robinson J;White O;Rikihisa Y;Tettelin H
通讯作者：
Tettelin H
影响因子：
14.9
作者：
Bagos, PG;Liakopoulos, TD;Hamodrakas, SJ
通讯作者：
Hamodrakas, SJ
影响因子：
9.4
作者：
Kanter, M;Mott, J;Rikihisa, Y
通讯作者：
Rikihisa, Y
影响因子：
3.1
作者：
Biswas, B;Vemulapalli, R;Dutta, SK
通讯作者：
Dutta, SK
影响因子：
2.1
作者：
Chae, JS;Pusterla, N;Madigan, JE
通讯作者：
Madigan, JE