Efficient Enrichment of Retinal DHA with Dietary Lysophosphatidylcholine-DHA: Potential Application for Retinopathies.
Efficient Enrichment of Retinal DHA with Dietary Lysophosphatidylcholine-DHA: Potential Application for Retinopathies.
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利用膳食溶血磷脂酰胆碱-DHA 高效富集视网膜 DHA：视网膜病变的潜在应用。
DOI：
10.3390/nu12103114
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发表时间：
2020-10-12
期刊：
影响因子：
5.9
通讯作者：
Subbaiah PV
中科院分区：
文献类型：
--
作者：
Sugasini D;Yalagala PCR;Subbaiah PV
虽然视网膜二十二碳六烯酸（DHA）减少是视网膜病的已知风险因素，但目前可用的ω-3脂肪酸补充剂（其作为三酰甘油（TAG）吸收）并不显著富集视网膜DHA。我们测试了这样的假设，即溶血磷脂酰胆碱（LPC）-DHA作为磷脂被吸收，将有效地增加视网膜DHA，因为在血液视网膜屏障的LPC特异性转运蛋白的存在。在正常大鼠中，LPC-DHA和在消化过程中产生LPC-DHA的二-DHA磷脂酰胆碱（PC）分别使视网膜DHA增加101%和45%，但在相同剂量（40 mg/kg，30天）下，TAG-DHA没有显著影响。在正常小鼠中，sn-1 DHA LPC和sn-2 DHA LPC均使视网膜DHA增加80%，但游离DHA没有影响。脂肪酶处理的磷虾油（含有LPC-DHA和LPC-EPA（二十碳五烯酸），但不含正常磷虾油（LPC很少），增加了视网膜DHA（+76%）和EPA（100倍）。然而，无论是否经过脂肪酶处理，鱼油都没有效果。这些研究表明，膳食LPC-DHA可以有效地增加视网膜DHA，但TAG-DHA或游离DHA不能。由于已知DHA可预防视网膜病变和其他眼部疾病，因此这项研究为预防/治疗这些疾病提供了一种新的营养方法。
Although decreased retinal docosahexaenoic acid (DHA) is a known risk factor for retinopathy, currently available omega-3 fatty acid supplements, which are absorbed as triacylglycerol (TAG), do not significantly enrich retinal DHA. We tested the hypothesis that lysophospahtidylcholine (LPC)-DHA which is absorbed as phospholipid, would efficiently increase retinal DHA because of the presence of LPC-specific transporter at the blood–retina barrier. In normal rats, LPC-DHA and di-DHA phosphatidylcholine (PC), which generates LPC-DHA during digestion, increased retinal DHA by 101% and 45%, respectively, but TAG-DHA had no significant effect at the same dose (40 mg/kg, 30 days). In normal mice, both sn-1 DHA LPC and sn-2 DHA LPC increased retinal DHA by 80%, but free DHA had no effect. Lipase-treated krill oil (which contains LPC-DHA and LPC-EPA (eicosapentaenoic acid), but not normal krill oil (which has little LPC), increased both retinal DHA (+76%) and EPA (100-fold). Fish oil, however, had no effect, whether lipase-treated or not. These studies show that retinal DHA can be efficiently increased by dietary LPC-DHA, but not by TAG-DHA or free DHA. Since DHA is known to be protective against retinopathy and other eye diseases, this study provides a novel nutraceutical approach for the prevention/treatment of these diseases.
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