In vivo quantitative study of sized-dependent transport and toxicity of single silver nanoparticles using zebrafish embryos.
In vivo quantitative study of sized-dependent transport and toxicity of single silver nanoparticles using zebrafish embryos.
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DOI：
10.1021/tx300021u
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发表时间：
2012-05-21
影响因子：
4.1
通讯作者：
Xu XH
中科院分区：
文献类型：
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作者：
Lee KJ;Browning LM;Nallathamby PD;Desai T;Cherukuri PK;Xu XH
纳米材料具有独特的物理化学性质（例如，小尺寸，高表面积体积比），并承诺广泛的应用，从设计高质量的消费产品到有效的疾病诊断和治疗。这些特性可能导致毒性作用，潜在地阻碍纳米技术的发展。在本研究中，我们合成并表征了纯化且稳定（非聚集）的银纳米粒子（Ag NPs，平均直径41.6±9.1 nm），并利用发育早期（卵裂期）的斑马鱼胚胎（关键的水生和生态物种）作为体内模型生物来研究Ag NPs的扩散和毒性。我们发现单个Ag NPs（直径30-72 nm）通过随机布朗运动通过绒毛膜孔被动扩散到胚胎中，并在胚胎的整个发育过程中（受精后120小时，hpf）停留在胚胎中。观察了NPs对胚胎发育的剂量和大小依赖性毒性作用，显示了NPs的生物相容性和毒性调节的可能性。在较低浓度的NPs（≤0.02 nM）下，75% - 91%的胚胎发育为正常斑马鱼。在NPs浓度较高（≥0.20 nM）时，100%的胚胎死亡。在（0.02 ~ 0.2 nM）浓度下，胚胎发育成各种变形的斑马鱼。我们定量分析了正常斑马鱼和变形斑马鱼在120 hpf下组织中嵌入的单个Ag NPs的数量和大小，结果表明变形斑马鱼比正常斑马鱼具有更多的较大NPs数量，并且具有大小依赖性的纳米毒性。通过与我们之前对较小的Ag NPs（11.6±3.5 nm）的研究比较，结果进一步证明了惊人的尺寸依赖性纳米毒性，在相同的摩尔浓度下，较大的Ag NPs（41.6±9.1 nm）比较小的Ag NPs（11.6±3.5 nm）更具毒性。
Nanomaterials possess distinctive physicochemical properties (e.g., small sizes, high surface area-to-volume ratios) and promise a wide variety of applications, ranging from design of high quality consumer products to effective disease diagnosis and therapy. These properties can lead to toxic effects, potentially hindering advance in nanotechnology. In this study, we have synthesized and characterized purified and stable (non-aggregation) silver nanoparticles (Ag NPs, 41.6±9.1 nm in average diameters), and utilized early-developing (cleavage-stage) zebrafish embryos (critical aquatic and eco- species) as in vivo model organisms to probe diffusion and toxicity of Ag NPs. We found that single Ag NPs (30–72 nm diameters) passively diffused into the embryos through chorionic pores via random Brownian motion and stayed inside the embryos throughout their entire development (120 hours-post-fertilization, hpf). Dose and size dependent toxic effects of the NPs on embryonic development were observed, showing the possibility of tuning biocompatibility and toxicity of the NPs. At lower concentrations of the NPs (≤ 0.02 nM), 75–91% of embryos developed to normal zebrafish. At the higher concentrations of NPs (≥ 0.20 nM), 100% of embryos became dead. At the concentrations in between (0.02–0.2 nM), embryos developed to various deformed zebrafish. Number and sizes of individual Ag NPs embedded in tissues of normal and deformed zebrafish at 120 hpf were quantitatively analyzed, showing deformed zebrafish with higher number of larger NPs than normal zebrafish, and size-dependent nanotoxicity. By comparing with our previous studies of smaller Ag NPs (11.6±3.5 nm), the results further demonstrate striking size-dependent nanotoxicity that, at the same molar concentration, the larger Ag NPs (41.6±9.1 nm) are more toxic than the smaller Ag NPs (11.6±3.5 nm).
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影响因子：
17.1
作者：
Lee, Kerry J.;Nallathamby, Prakash D.;Xu, Xiao-Hong Nancy
通讯作者：
Xu, Xiao-Hong Nancy
影响因子：
3.4
作者：
KUSUMI, A;SAKO, Y;YAMAMOTO, M
通讯作者：
YAMAMOTO, M
影响因子：
6.7
作者：
Browning LM;Lee KJ;Huang T;Nallathamby PD;Lowman JE;Xu XH
通讯作者：
Xu XH
影响因子：
17.1
作者：
AshaRani, P. V.;Mun, Grace Low Kah;Valiyaveettil, Suresh
通讯作者：
Valiyaveettil, Suresh
影响因子：
38.3
作者：
通讯作者：
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