Identification and characterisation of a novel adhesin Ifp in Yersinia pseudotuberculosis.
Identification and characterisation of a novel adhesin Ifp in Yersinia pseudotuberculosis.
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DOI：
10.1186/1471-2180-11-85
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发表时间：
2011-04-28
期刊：
影响因子：
4.2
通讯作者：
Wren BW
中科院分区：
文献类型：
--
作者：
Strong PC;Hinchliffe SJ;Patrick H;Atkinson S;Champion OL;Wren BW
为了确定假结核耶尔森氏菌的新毒力决定因素，对其基因组与鼠疫耶尔森氏菌的基因组进行了比较。这揭示了鼠疫耶尔森氏菌中的数十个假基因，这些假基因在假结核耶尔森氏菌中仍然具有功能，并且可能在肠道生活方式中很重要。其中一个基因，假结核杆菌 IP32953 基因组序列中的 YPTB1572，编码一种与 invasin（一种经典的粘附/侵袭蛋白）和 intimin（来自肠病原性 (EPEC) 和肠出血性 (EHEC) 大肠杆菌的粘附和擦除蛋白）相似的蛋白质。我们将其命名为 YPTB1572 Ifp（Intimin 家族蛋白），并表明它能够直接与人 HEp-2 上皮细胞结合。 intimin C 末端区域中对 EPEC 和 EHEC 功能至关重要的半胱氨酸和色氨酸残基在 Ifp 中是保守的。蛋白质结合发生在 HEp-2 细胞表面的不同焦点处，并且可以通过蛋白质 C 末端的单个半胱氨酸残基的突变来破坏。使用 lux 报告基因构建体进行的时间表达分析表明，与 invasin 相比，ifp 在 37°C 的对数晚期表达，表明 Ifp 是晚期粘附素。 ifp 定义的突变体显示出对 HEp-2 细胞的粘附力降低，并且在大蜡螟感染模型中减弱。一种新的假结核杆菌粘附素已被鉴定和表征。该 Ifp 是 invasin/intimin 外膜粘附素家族的新成员。
In order to identify new virulence determinants in Y. pseudotuberculosis a comparison between its genome and that of Yersinia pestis was undertaken. This reveals dozens of pseudogenes in Y. pestis, which are still putatively functional in Y. pseudotuberculosis and may be important in the enteric lifestyle. One such gene, YPTB1572 in the Y. pseudotuberculosis IP32953 genome sequence, encodes a protein with similarity to invasin, a classic adhesion/invasion protein, and to intimin, the attaching and effacing protein from enteropathogenic (EPEC) and enterohaemorraghic (EHEC) Escherichia coli. We termed YPTB1572 Ifp (Intimin family protein) and show that it is able to bind directly to human HEp-2 epithelial cells. Cysteine and tryptophan residues in the C-terminal region of intimin that are essential for function in EPEC and EHEC are conserved in Ifp. Protein binding occurred at distinct foci on the HEp-2 cell surface and can be disrupted by mutation of a single cysteine residue at the C-terminus of the protein. Temporal expression analysis using lux reporter constructs revealed that ifp is expressed at late log phase at 37°C in contrast to invasin, suggesting that Ifp is a late stage adhesin. An ifp defined mutant showed a reduction in adhesion to HEp-2 cells and was attenuated in the Galleria mellonella infection model. A new Y. pseudotuberculosis adhesin has been identified and characterised. This Ifp is a new member in the family of invasin/intimin outer membrane adhesins.
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影响因子：
3.1
作者：
Clark, MA;Hirst, BH;Jepson, MA
通讯作者：
Jepson, MA
影响因子：
3.1
作者：
FRANKEL, G;CANDY, DCA;DOUGAN, G
通讯作者：
DOUGAN, G
影响因子：
64.5
作者：
ISBERG, RR;LEONG, JM
通讯作者：
LEONG, JM
影响因子：
3.1
作者：
BLISKA, JB;COPASS, MC;FALKOW, S
通讯作者：
FALKOW, S
影响因子：
3.2
作者：
GUZMAN, LM;BELIN, D;BECKWITH, J
通讯作者：
BECKWITH, J