Morphine and galectin-1 modulate HIV-1 infection of human monocyte-derived macrophages.
Morphine and galectin-1 modulate HIV-1 infection of human monocyte-derived macrophages.
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DOI：
10.4049/jimmunol.1102276
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发表时间：
2012-04-15
期刊：
影响因子：
--
通讯作者：
Schwartz SA
中科院分区：
文献类型：
--
作者：
Reynolds JL;Law WC;Mahajan SD;Aalinkeel R;Nair B;Sykes DE;Mammen MJ;Yong KT;Hui R;Prasad PN;Schwartz SA
吗啡是一种被广泛滥用的成瘾性药物，可以调节免疫功能。巨噬细胞是HIV-1的主要储存库；因此，它们不仅在这种疾病的发展中发挥作用，而且影响疾病进展的整体过程。半乳糖凝集素-1是β-半乳糖苷结合凝集素家族的一员，是可溶性粘附分子，在HIV-1病毒粘附过程中介导直接的细胞-病原体相互作用。由于药物滥用流行与HIV-1流行密切相关，我们提出吗啡诱导的半乳糖凝集素-1表达增加可能调节人类单核细胞源性巨噬细胞（MDM）的HIV-1感染。在这里，我们发现半乳糖凝集素-1基因和蛋白的表达在吗啡的孵育下增强。证实先前的研究，吗啡单独或半凝集素-1单独增加MDM的HIV-1感染。外源性半乳糖凝集素-1和吗啡同时孵育可增强MDM的HIV-1感染。我们利用纳米技术方法，使用金纳米棒-半乳糖凝集素-1 siRNA复合物（纳米复合物）来抑制半乳糖凝集素-1的基因表达。我们发现纳米丛沉默了半乳糖凝集素-1的基因表达，并且纳米丛逆转了吗啡对半乳糖凝集素-1表达的影响。此外，纳米复合物的存在降低了吗啡对HIV-1感染的影响。
Morphine is a widely abused, addictive drug that modulates immune function. Macrophages are a primary reservoir of HIV-1; therefore, they not only play a role in the development of this disease but also impact the overall course of disease progression. Galectin-1 is a member of a family of β-galactoside-binding lectins that are soluble adhesion molecules and that mediate direct cell-pathogen interactions during HIV-1 viral adhesion. Since the drug abuse epidemic and the HIV-1 epidemic are closely interrelated we propose that increased expression of galectin-1 induced by morphine may modulate HIV-1 infection of human monocytes-derived macrophages (MDM). Here, we show that galectin-1 gene and protein expression are potentiated by incubation with morphine. Confirming previous studies, morphine alone or galectin-1 alone enhance HIV-1 infection of MDM. Concomitant incubation with exogenous galectin-1 and morphine potentiated HIV-1 infection of MDM. We utilized a nanotechnology approach that uses gold nanorod-galectin-1 siRNA complexes (nanoplexes) to inhibit gene expression for galectin-1. We found that nanoplexes silenced gene expression for galectin-1 and the nanoplexes reversed the effects of morphine on galectin-1 expression. Furthermore, the effects of morphine on HIV-1 infection were reduced in the presence of the nanoplex.
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影响因子：
3.3
作者：
Bergamaschi A;Pancino G
通讯作者：
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作者：
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通讯作者：
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