Spontaneous formation of two-dimensional and three-dimensional cholesterol crystals in single hydrated lipid bilayers.
Spontaneous formation of two-dimensional and three-dimensional cholesterol crystals in single hydrated lipid bilayers.
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在单个水合脂质双层中自发形成二维和三维胆固醇晶体。
DOI：
10.1016/j.bpj.2012.05.025
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发表时间：
2012
影响因子：
3.4
通讯作者：
L. Addadi
中科院分区：
文献类型：
--
作者：
R. Ziblat;Iael Fargion;L. Leiserowitz;L. Addadi
对不同浓度的神经酰胺/胆固醇/1-棕榈酰-2-油酰-sn-甘油-3-磷酸腺苷的单层和单层水合x射线衍射进行了测量。由于对立的脂质小叶之间的相互作用，相对于相应的单层，在双层内形成的晶体域的相和结构行为存在实质性差异。根据脂质组成，这些相互作用导致相分离和胆固醇晶体的形成。在37°C下，用识别胆固醇有序分子阵列的特异性抗体进一步对胆固醇和神经酰胺/胆固醇混合相进行免疫标记。先前的研究表明，胆固醇可能在人造膜中成核，形成厚的二维双层晶体。这里的研究表明胆固醇进一步生长成三维晶体。我们相信，这些结果可能为动脉粥样硬化炎症早期阶段胆固醇晶体的形成提供进一步的见解。
Grazing incidence x-ray diffraction measurements were performed on single hydrated bilayers and monolayers of Ceramide/Cholesterol/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocyholine at varying concentrations. There are substantial differences in the phase and structure behavior of the crystalline domains formed within the bilayers relative to the corresponding monolayers, due to interactions between the opposing lipid leaflets. Depending on the lipid composition, these interactions lead to phase separation and formation of cholesterol crystals. The cholesterol and ceramide/cholesterol mixed phases were further characterized at 37°C by immunolabeling with specific antibodies recognizing ordered molecular arrays of cholesterol. Previous studies have shown that cholesterol may nucleate in artificial membranes to form thick two-dimensional bilayer crystals. The study herein demonstrates further growth of cholesterol into three-dimensional crystals. We believe that these results may provide further insight into the formation of cholesterol crystals in early stages of atherosclerosis inflammation.
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影响因子：
15.9
作者：
SMALL, DM;BOND, MG;SAWYER, JK
通讯作者：
SAWYER, JK
DOI：
10.1016/j.bbalip.2008.09.002
发表时间：
2008-11
影响因子：
4.8
作者：
Goni, Felix M.;Alonso, Alicia;Bagatolli, Luis A.;Brown, Rhoderick E.;Marsh, Derek;Prieto, Manuel;Thewalt, Jenifer L.
通讯作者：
Thewalt, Jenifer L.
影响因子：
3.4
作者：
Veatch, SL;Keller, SL
通讯作者：
Keller, SL
不对称脂质双层中的域耦合。
影响因子：
3.4
作者：
Kiessling, Volker;Wan, Chen;Tamm, Lukas K.
通讯作者：
Tamm, Lukas K.