Transcriptome profiling reveals transcriptional and alternative splicing regulation in the early embryonic development of hair follicles in the cashmere goat
Transcriptome profiling reveals transcriptional and alternative splicing regulation in the early embryonic development of hair follicles in the cashmere goat
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转录组分析揭示了绒山羊毛囊早期胚胎发育中的转录和选择性剪接调控
DOI：
10.1038/s41598-019-54315-7
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发表时间：
2019-11
影响因子：
4.6
通讯作者：
Jinquan Li
中科院分区：
文献类型：
--
作者：
Yanjun Zhang;Lele Wang;Zhen Li;Dong Chen;Wenjing Han;Zhihong Wu;Fangzheng Shang;Erhan Hai;Yaxun Wei;Rui Su;Zhihong Liu;Ruijun Wang;Zhiying Wang;Yanhong Zhao;Zhixin Wang;Yi Zhang;Jinquan Li
绒山羊的底毛纤维来源于次级毛囊，具有很高的商业价值。然而，很少有研究集中在山羊胚胎原发性和继发性HF的发生和发展的分子细节。在这项研究中，收集胚胎第45、55和65天（E45、E55和E65）的皮肤样品并制备用于RNA测序（RNA-seq）。通过对差异表达基因（DEG）功能通路的分析，我们发现HF可能起源于E55-E65。经典信号通路中的大多数关键基因，包括WNT、TGF-β、FGF、Hedgehog、NOTCH和其他因子，从E55到E65显示出明显的表达变化。我们，第一次，探讨了选择性剪接（AS）的改变，这三个阶段之间表现出不同的模式。AS调控基因的功能途径与HF的发生有关。通过比较已发表的E60、E120和新生儿（NB）阶段的RNA-seq样本，我们发现大多数WNT/β-catenin信号传导基因在HF发生的起始中很重要，而其他因素包括FOXN 1、GATA 3和DLX 3可能对HF发生具有一致的影响。我们的研究支持胚胎HF起始的时间点，并确定了具有胚胎HF起始和发育的潜在功能的基因。我们进一步探讨了AS在HF发生中的潜在调节作用，扩展了我们对HF发生的分子机制的认识。
The undercoat fiber of the cashmere goat, from the secondary hair follicle (HF), possesses commercial value. However, very few studies have focused on the molecular details of primary and secondary HF initiation and development in goat embryos. In this study, skin samples at embryonic day 45, 55, and 65 (E45, E55, and E65) were collected and prepared for RNA sequencing (RNA-seq). We found that the HF probably initiated from E55 to E65 by analyzing the functional pathways of differentially expressed genes (DEGs). Most key genes in canonical signaling pathways, including WNT, TGF-β, FGF, Hedgehog, NOTCH, and other factors showed clear expression changes from E55 to E65. We, for the first time, explored alternative splicing (AS) alterations, which showed distinct patterns among these three stages. Functional pathways of AS-regulated genes showed connections to HF development. By comparing the published RNA-seq samples from the E60, E120, and newborn (NB) stages, we found the majority of WNT/β-catenin signaling genes were important in the initiation of HF development, while other factors includingFOXN1,GATA3, andDLX3may have a consistent influence on HF development. Our investigation supported the time points of embryonic HF initiation and identified genes that have potential functions of embryonic HF initiation and development. We further explored the potential regulatory roles of AS in HF initiation, which extended our knowledge about the molecular mechanisms of HF development.
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