Expression profile of MicroRNAs in young stroke patients.
Expression profile of MicroRNAs in young stroke patients.
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DOI：
10.1371/journal.pone.0007689
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发表时间：
2009-11-02
期刊：
影响因子：
3.7
通讯作者：
Jeyaseelan K
中科院分区：
文献类型：
--
作者：
Tan KS;Armugam A;Sepramaniam S;Lim KY;Setyowati KD;Wang CW;Jeyaseelan K
目前用于脑缺血诊断和预后的方法仍需进一步改进。Micro-RNA（小的非编码RNA）最近被报道为癌症和糖尿病等疾病的有用生物标志物。因此，我们从外周血中进行microRNA（miRNA）分析，以检测和识别缺血性卒中的特征模式。根据吐司分类法，对年龄在18-49岁之间的缺血性卒中患者（根据世界卫生组织临床标准进行表征）进行进一步分类，a）大血管动脉粥样硬化[n = 8] B）小血管疾病[n = 3] c）血栓栓塞[n = 5] d）原因不明[n = 3]。 采用改良兰金量表（mRS）评价患者在门诊采血时的功能状态。来自正常（n = 5）个体的血液样品用作对照。 对从全血提取的总RNA进行miRNA谱分析和实时PCR分析。与正常对照相比，与内皮/血管功能、红细胞生成、血管生成和神经功能有关的miRNA显示出差异表达谱。有趣的是，在我们的miRNA谱中也发现了参与缺氧条件的miRNA。外周血miRNAs及其表达谱可作为缺血性脑卒中诊断和预后的生物标志物。在通常被认为是神经系统稳定的患者中，即使在中风发作后几个月，也可以检测到失调的miRNA。
The methods currently available for diagnosis and prognosis of cerebral ischaemia still require further improvements. Micro-RNAs (small non-coding RNAs) have been recently reported as useful biomarkers in diseases such as cancer and diabetes. We therefore carried out microRNA (miRNA) profiling from peripheral blood to detect and identify characteristic patterns in ischaemic stroke. The ischaemic stroke patients aged between 18–49 years, characterized based on World Health Organization clinical criteria were further classified according to TOAST classification, a) Large-vessel atherosclerosis [n = 8] b) Small-vessel disease [n = 3] c) Cardioembolism [n = 5] d) Undetermined cause [n = 3]. The patients' functional status at the time of blood sampling (at the outpatient clinics) was evaluated with the modified Rankin Scale (mRS). Blood samples from normal (n = 5) individuals were used as controls. Total RNA extracted from whole blood was subjected to miroRNA profiling and real-time PCR analysis. miRNAs that are implicated in the endothelial/vascular function, erythropoiesis, angiogenesis and neural function showed differential expression profile as compared to the normal control. Interestingly, miRNAs that are involved in hypoxic conditions have also been found in our miRNA profiles. We demonstrate that the peripheral blood miRNAs and their profiles can be developed as biomarkers in diagnosis and prognosis of cerebral ischaemic stroke. The dysregulated miRNAs have been detectable even after several months from the onset of stroke in what is usually regarded as neurologically stable patients.
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DOI：
10.1038/jcbfm.2008.157
发表时间：
2009-04
期刊：
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
影响因子：
--
作者：
通讯作者：
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影响因子：
6.4
作者：
Nelson, Peter T.;Wang, Wang-Xia;Rajeev, Bernard W.
通讯作者：
Rajeev, Bernard W.
microRNA表达模式在人肝内胆管癌中的作用。
影响因子：
25.7
作者：
Chen, Lei;Yan, He-Xin;Wang, Hong-Yang
通讯作者：
Wang, Hong-Yang
影响因子：
64.8
作者：
Johnnidis, Jonathan B.;Harris, Marian H.;Camargo, Fernando D.
通讯作者：
Camargo, Fernando D.
血清中 microRNA 的表征：一类用于诊断癌症和其他疾病的新型生物标志物
影响因子：
44.1
作者：
Chen, Xi;Ba, Yi;Zhang, Chen-Yu
通讯作者：
Zhang, Chen-Yu