Analysis of sea-island cotton and upland cotton in response to Verticillium dahliae infection by RNA sequencing.
Analysis of sea-island cotton and upland cotton in response to Verticillium dahliae infection by RNA sequencing.
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DOI：
10.1186/1471-2164-14-852
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发表时间：
2013-12-05
期刊：
影响因子：
4.4
通讯作者：
Cai Y
中科院分区：
文献类型：
--
作者：
Sun Q;Jiang H;Zhu X;Wang W;He X;Shi Y;Yuan Y;Du X;Cai Y
棉花黄萎病是一种严重的土传维管束病害，每年造成巨大的经济损失。然而，由于陆地棉抗病品种的缺乏，其抗病的分子机制，尤其是对黄萎病的抗性机制尚不清楚。通过对海岛棉和陆地棉的比较，利用RNA-seq方法研究了棉花对不同小种黄萎病的防御反应的分子机制。共获得77,212个非特征基因，对这些非基因基因进行BLAST搜索，并使用GO和KO数据库进行注释。六组数字基因表达数据被映射到参考转录组。比较了侵染黄萎病棉花和未侵染棉花的基因表达谱，共鉴定出44个差异表达基因。在苯丙氨酸代谢途径基因方面，陆地棉上调了羟基肉桂酰基转移酶基因(HCT)，而海岛棉上调了PAL、4CL、CAD、CCoAOMT和COMT。海岛棉和陆地棉分别感染大丽弧菌V991和大丽弧菌D07038后，几乎没有发现该途径的差异表达基因。我们在转录水平上的全面基因表达数据将有助于阐明棉花对大丽叶枯病菌的防御反应的分子机制。通过识别每种类型棉花对大丽弧菌的防御反应相关基因，我们的数据不仅为研究人员提供了新的分子信息，而且有助于加快棉花防御相关基因的研究。
Cotton Verticillium wilt is a serious soil-borne vascular disease that causes great economic loss each year. However, due to the lack of resistant varieties of upland cotton, the molecular mechanisms of resistance to this disease, especially to the pathogen Verticillium dahliae, remain unclear. We used the RNA-seq method to research the molecular mechanisms of cotton defence responses to different races of Verticillium dahliae by comparing infected sea-island cotton and upland cotton. A total of 77,212 unigenes were obtained, and the unigenes were subjected to BLAST searching and annotated using the GO and KO databases. Six sets of digital gene expression data were mapped to the reference transcriptome. The gene expression profiles of cotton infected with Verticillium dahliae were compared to those of uninfected cotton; 44 differentially expressed genes were identified. Regarding genes involved in the phenylalanine metabolism pathway, the hydroxycinnamoyl transferase gene (HCT) was upregulated in upland cotton whereas PAL, 4CL, CAD, CCoAOMT, and COMT were upregulated in sea-island cotton. Almost no differentially expressed genes in this pathway were identified in sea-island cotton and upland cotton when they were infected with V. dahliae V991 and V. dahliae D07038, respectively. Our comprehensive gene expression data at the transcription level will help elucidate the molecular mechanisms of the cotton defence response to V. dahliae. By identifying the genes involved in the defence response of each type of cotton to V. dahliae, our data not only provide novel molecular information for researchers, but also help accelerate research on genes involved in defences in cotton.
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