Increased Risky Choice and Reduced CHRNB2 Expression in Adult Male Rats Exposed to Nicotine Vapor.
Increased Risky Choice and Reduced CHRNB2 Expression in Adult Male Rats Exposed to Nicotine Vapor.
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DOI：
10.3390/ijms23031231
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发表时间：
2022-01-22
影响因子：
5.6
通讯作者：
Mendez IA
中科院分区：
文献类型：
--
作者：
Giner P;Maynez-Anchondo L;Liley AE;Uribe KP;Frietze GA;Simon NW;Mendez IA
虽然尼古丁使用的认知增强作用已经得到了很好的证明，但它也被证明会损害决策。这项研究的目的是确定暴露于尼古丁蒸汽是否会增加风险决策。该研究还旨在研究尼古丁蒸汽暴露对大脑中胆碱能和多巴胺能受体编码基因表达的可能长期影响。将32只成年雄性Sprague道利大鼠暴露于24 mg/mL尼古丁蒸汽或溶剂对照，随后立即进行连续10天的概率折扣任务测试。在10天蒸汽暴露后54天，处死动物，并使用RT-PCR分析编码α4和β2胆碱能受体亚单位以及多巴胺D1和D2受体的基因表达。暴露于尼古丁蒸汽会导致风险选择的立即和短暂增加。基因表达分析发现，相对于溶剂对照组，先前暴露于尼古丁蒸汽的大鼠的丘脑核核心中的CHRNB 2和DRD 1以及内侧前额叶皮层中的CHRNB 2和DRD 2显著降低。结果提供了尼古丁蒸汽暴露的负面认知影响的数据，并确定了可能受重复使用影响的胆碱能和多巴胺能机制。
While the cognitive enhancing effects of nicotine use have been well documented, it has also been shown to impair decision making. The goal of this study was to determine if exposure to nicotine vapor increases risky decision making. The study also aims to investigate possible long-term effects of nicotine vapor exposure on the expression of genes coding for cholinergic and dopaminergic receptors in brain. Thirty-two adult male Sprague Dawley rats were exposed to 24 mg/mL nicotine vapor or vehicle control, immediately followed by testing in the probability discounting task for 10 consecutive days. Fifty-four days after the 10-day vapor exposure, animals were sacrificed and expression of genes coding for the α4 and β2 cholinergic receptor subunits, and dopamine D1 and D2 receptors, were analyzed using RT-PCR. Exposure to nicotine vapor caused an immediate and transient increase in risky choice. Analyses of gene expression identified significant reductions in CHRNB2 and DRD1 in the nucleus accumbens core and CHRNB2 and DRD2 in the medial prefrontal cortex of rats previously exposed to nicotine vapor, relative to vehicle controls. Results provide data on the negative cognitive effects of nicotine vapor exposure and identify cholinergic and dopaminergic mechanisms that may affected with repeated use.
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影响因子：
33.9
作者：
Gentzke, Andrea S.;Creamer, MeLisa;King, Brian A.
通讯作者：
King, Brian A.
影响因子：
3.3
作者：
Faure, Alexis;Zoratto, Francesca;Adriani, Walter
通讯作者：
Adriani, Walter
影响因子：
3.4
作者：
Gabriel, Daniel B. K.;Liley, Anna E.;Simon, Nicholas W.
通讯作者：
Simon, Nicholas W.
尼古丁制剂会影响蒸汽自我给药的小鼠模型中与增强相关的行为。
影响因子：
4.2
作者：
Henderson BJ;Cooper SY
通讯作者：
Cooper SY
影响因子：
10.6
作者：
Day, Jeremy J.;Jones, Joshua L.;Wightman, R. Mark;Carelli, Regina M.
通讯作者：
Carelli, Regina M.