Whole-Brain Monosynaptic Afferent Projections to the Cholecystokinin Neurons of the Suprachiasmatic Nucleus.
Whole-Brain Monosynaptic Afferent Projections to the Cholecystokinin Neurons of the Suprachiasmatic Nucleus.
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全脑单突触传入投射到视交叉上核的胆囊收缩素神经元。
DOI：
10.3389/fnins.2018.00807
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发表时间：
2018
影响因子：
4.3
通讯作者：
Huang ZL
中科院分区：
文献类型：
--
作者：
Yuan XS;Wei HH;Xu W;Wang L;Qu WM;Li RX;Huang ZL
视交叉上核（SCN）是驱动生理行为昼夜节律的主要起搏器。SCN由表达神经肽的不同神经元组成，所述神经肽包括精氨酸加压素（AVP）、血管活性肠肽（VIP）、胃泌素释放肽（GRP）、胆囊收缩素（CCK）等。AVP、VIP和GRP神经元接收来自视网膜的光刺激以使内源性生物钟与太阳日同步，而CCK神经元不直接受视网膜神经节细胞支配，可能参与昼夜节律钟的非光调节。为了更好地了解CCK神经元在非光昼夜节律中的功能，阐明SCN中CCK神经元的直接传入输入至关重要。在这里，我们利用了最近开发的狂犬病病毒和Cre/loxP为基础的，细胞类型特异性，逆行追踪系统映射和定量分析SCN CCK神经元的全脑单突触输入。我们发现SCN CCK神经元接受来自29个脑核团的直接输入。在这些核团中，下丘脑室旁核（PVH），丘脑室旁核（PVT），视上核（SON），下丘脑腹内侧核，和其他七个核团发送大量的输入CCK神经元。中等输入起源于齿状回，下丘脑室周围核，和其他五个核团。CCK神经元的少数输入来自眶额叶皮质、边缘前皮质、扣带回皮质、屏状核和其他7个核团。此外，SCN CCK神经元优先支配的AVP神经元的同侧PVH和SON，而不是对侧的对应，而对侧PVT发送更多的投射到CCK神经元比同侧的对应。总之，这些结果扩大了我们对小鼠SCN CCK神经元的特异性神经支配的认识，并为进一步研究CCK神经元的功能提供了重要的指示。
The suprachiasmatic nucleus (SCN) is the principal pacemaker driving the circadian rhythms of physiological behaviors. The SCN consists of distinct neurons expressing neuropeptides, including arginine vasopressin (AVP), vasoactive intestinal polypeptide (VIP), gastrin-releasing peptide (GRP), cholecystokinin (CCK), and so on. AVP, VIP, and GRP neurons receive light stimulation from the retina to synchronize endogenous circadian clocks with the solar day, whereas CCK neurons are not directly innervated by retinal ganglion cells and may be involved in the non-photic regulation of the circadian clock. To better understand the function of CCK neurons in non-photic circadian rhythm, it is vital to clarify the direct afferent inputs to CCK neurons in the SCN. Here, we utilized a recently developed rabies virus- and Cre/loxP-based, cell type-specific, retrograde tracing system to map and quantitatively analyze the whole-brain monosynaptic inputs to SCN CCK neurons. We found that SCN CCK neurons received direct inputs from 29 brain nuclei. Among these nuclei, paraventricular nucleus of the hypothalamus (PVH), paraventricular nucleus of the thalamus (PVT), supraoptic nucleus (SON), ventromedial nucleus of the hypothalamus, and seven other nuclei sent numerous inputs to CCK neurons. Moderate inputs originated from the zona incerta, periventricular hypothalamic nucleus, and five other nuclei. A few inputs to CCK neurons originated from the orbital frontal cortex, prelimbic cortex, cingulate cortex, claustrum, and seven other nuclei. In addition, SCN CCK neurons were preferentially innervated by AVP neurons of the ipsilateral PVH and SON rather than their contralateral counterpart, whereas the contralateral PVT sent more projections to CCK neurons than to its ipsilateral counterpart. Taken together, these results expand our knowledge of the specific innervation to mouse SCN CCK neurons and provide an important indication for further investigations on the function of CCK neurons.
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影响因子：
16.2
作者：
Dhillon, H;Zigman, JM;Lowelll, BB
通讯作者：
Lowelll, BB
影响因子：
4.8
作者：
Flanagan-Cato, Loretta M.;Fluharty, Steven J.;LaBelle, Denise R.
通讯作者：
LaBelle, Denise R.
影响因子：
4.8
作者：
Hume, Catherine;Sabatier, Nancy;Menzies, John
通讯作者：
Menzies, John
DOI：
10.1152/ajpregu.00355.2002
发表时间：
2003-03-01
影响因子：
2.8
作者：
Buxton, OM;Lee, CW;Van Cauter, E
通讯作者：
Van Cauter, E
影响因子：
3
作者：
Hsu DT;Kirouac GJ;Zubieta JK;Bhatnagar S
通讯作者：
Bhatnagar S