Altered intestinal microbiome and metabolome correspond to the clinical outcome of sepsis.
Altered intestinal microbiome and metabolome correspond to the clinical outcome of sepsis.
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DOI：
10.1186/s13054-023-04412-x
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发表时间：
2023-03-28
期刊：
影响因子：
15.1
通讯作者：
Mao, Enqiang
中科院分区：
文献类型：
--
作者：
Sun, Silei;Wang, Daosheng;Dong, Danfeng;Xu, Lili;Xie, Mengqi;Wang, Yihui;Ni, Tongtian;Jiang, Weisong;Zhu, Xiaojuan;Ning, Ning;Sun, Qian;Zhao, Shuyuan;Li, Mengjiao;Chen, Peili;Yu, Meiling;Li, Jian;Chen, Erzhen;Zhao, Bing;Peng, Yibing;Mao, Enqiang
肠道微生物群在脓毒症的发展过程中起着关键作用。然而，肠道微生物区系及其代谢产物参与脓毒症过程的具体机制仍不清楚，这限制了其翻译应用。在这项研究中，我们使用微生物组和非靶向代谢组学相结合的方法来分析入院时登记的脓毒症患者的粪便样本，然后筛选出可能在疾病转归中发挥重要作用的微生物区系、代谢物和潜在的信号通路。最后，通过对脓毒症动物模型的微生物组和转录组分析验证了上述结果。败血症患者表现出共生菌群破坏和肠球菌丰度增加，这在动物实验中得到了证实。此外，类杆菌感染负担高的患者，特别是普通杆菌，急性生理学和慢性健康评估II得分更高，在重症监护病房停留的时间更长。CLP大鼠肠道转录组分析表明，肠球菌和类杆菌具有与差异表达基因相关的不同图谱，表明这两种细菌在脓毒症中的作用明显不同。此外，与健康对照组相比，脓毒症患者表现出肠道氨基酸代谢的紊乱；即色氨酸代谢与微生物区系的改变和脓毒症的严重程度密切相关。肠道微生物和代谢特征的改变与脓毒症的进展相对应。我们的发现可能有助于预测脓毒症早期患者的临床结果，并为探索新的治疗方法提供翻译依据。网上版载有补充材料，可在10.1186/s13054-023-04412-x查阅。
The gut microbiome plays a pivotal role in the progression of sepsis. However, the specific mechanism of gut microbiota and its metabolites involved in the process of sepsis remains elusive, which limits its translational application. In this study, we used a combination of the microbiome and untargeted metabolomics to analyze stool samples from patients with sepsis enrolled at admission, then microbiota, metabolites, and potential signaling pathways that might play important roles in disease outcome were screened out. Finally, the above results were validated by the microbiome and transcriptomics analysis in an animal model of sepsis. Patients with sepsis showed destruction of symbiotic flora and elevated abundance of Enterococcus, which were validated in animal experiments. Additionally, patients with a high burden of Bacteroides, especially B. vulgatus, had higher Acute Physiology and Chronic Health Evaluation II scores and longer stays in the intensive care unit. The intestinal transcriptome in CLP rats illustrated that Enterococcus and Bacteroides had divergent profiles of correlation with differentially expressed genes, indicating distinctly different roles for these bacteria in sepsis. Furthermore, patients with sepsis exhibited disturbances in gut amino acid metabolism compared with healthy controls; namely, tryptophan metabolism was tightly related to an altered microbiota and the severity of sepsis. Alterations in microbial and metabolic features in the gut corresponded with the progression of sepsis. Our findings may help to predict the clinical outcome of patients in the early stage of sepsis and provide a translational basis for exploring new therapies. The online version contains supplementary material available at 10.1186/s13054-023-04412-x.
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影响因子：
5.2
作者：
Engels C;Ruscheweyh HJ;Beerenwinkel N;Lacroix C;Schwab C
通讯作者：
Schwab C
DOI：
10.1126/science.aam9949
发表时间：
2017-08-11
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Byndloss MX;Olsan EE;Rivera-Chávez F;Tiffany CR;Cevallos SA;Lokken KL;Torres TP;Byndloss AJ;Faber F;Gao Y;Litvak Y;Lopez CA;Xu G;Napoli E;Giulivi C;Tsolis RM;Revzin A;Lebrilla CB;Bäumler AJ
通讯作者：
Bäumler AJ
双歧杆菌影响 DNFB 诱导的特应性皮炎小鼠肠道微生物组成、SCFA 代谢和免疫力之间的相关性
影响因子：
5.2
作者：
Fang, Zhifeng;Li, Lingzhi;Chen, Wei
通讯作者：
Chen, Wei
影响因子：
3.9
作者：
Derkacz A;Olczyk P;Olczyk K;Komosinska-Vassev K
通讯作者：
Komosinska-Vassev K
影响因子：
12.2
作者：
Agudelo-Ochoa GM;Valdés-Duque BE;Giraldo-Giraldo NA;Jaillier-Ramírez AM;Giraldo-Villa A;Acevedo-Castaño I;Yepes-Molina MA;Barbosa-Barbosa J;Benítez-Paéz A
通讯作者：
Benítez-Paéz A