Muscle specification in the Xenopus laevis gastrula-stage embryo.

Muscle specification in the Xenopus laevis gastrula-stage embryo.
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非洲爪蟾原肠胚阶段胚胎的肌肉规格。

DOI:
10.1002/dvdy.20451
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发表时间:
2005
期刊:
Developmental dynamics : an official publication of the American Association of Anatomists.
影响因子:
--
通讯作者:
Domingo,CarmenR
Domingo,CarmenR
中科院分区:
--
文献类型:
--
作者:
Wunderlich,Kathleen;Gustin,JeanK;Domingo,CarmenR

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非洲爪蟾原肠胚的最新命运图显示,围绕囊胚孔的中胚层组织产生肌肉(Keller [1991] Methods Cell Biol 36:61-113; Lane和Smith [1999] Development 126:423-434)。与早期数据存在显着偏差的是,新的地图表明原肠胚腹侧一半的细胞是大部分躯干体节的前体。然而,这些后部体节直到蝌蚪阶段(阶段38-44)才形成。因此,我们着手确定肌肉规格内腹侧一半的原肠胚的时间。我们的方法是从原肠胚阶段的胚胎中产生一系列组织外植体,然后将它们培养到第28阶段(尾芽)或第44阶段(蝌蚪)。在每个终点,使用肌肉特异性抗体评估外植体中肌肉的存在。有趣的是,我们发现在腹侧外植体中检测到肌肉组织。然而,这些外植体必须培养到蝌蚪阶段。这也许并不意外,因为这是该组织通常产生肌肉的点。我们进一步表明,肌肉规格的退化边缘区不会改变原肠胚形成的过程中。总之,这些结果表明,背侧信号从中线在原肠胚形成过程中是没有必要的肌肉规格的腹侧一半的渐缩边缘区。发展动力学233:1348-1358,2005年。© 2005 Wiley利斯公司
Recent fate maps of theXenopus laevisgastrula show that mesodermal tissue surrounding the blastopore gives rise to muscle (Keller [1991] Methods Cell Biol 36:61–113; Lane and Smith [1999] Development 126:423–434). In a significant deviation from earlier data, the new maps demonstrate that cells in the ventral half of the gastrula are precursors to a significant portion of trunk somites. However, these posterior somites are not formed until tadpole stages (stages 38–44). We therefore set out to determine the timing of muscle specification within the ventral half of the gastrula. Our approach was to generate a series of tissue explants from gastrula‐stage embryos and then culture them to either stage 28 (tailbud) or stage 44 (tadpole). At each endpoint, the presence of muscle in explants was assessed with a muscle‐specific antibody. Interestingly, we found that muscle tissue is detected in ventral explants. However, these explants must be cultured to the tadpole stage. This is perhaps not unexpected, as this is the point at which this tissue normally gives rise to muscle. We further show that muscle specification of the involuting marginal zone does not change over the course of gastrulation. Together, these results suggest that dorsalizing signals emanating from the midline during gastrulation are not necessary for muscle specification of the ventral half of the involuting marginal zone. Developmental Dynamics 233:1348–1358, 2005. © 2005 Wiley‐Liss, Inc.
DOI: 10.1111/j.1432-0436.1985.tb00287.x
发表时间: 1985-03
期刊: Differentiation; research in biological diversity
影响因子: --
作者:
J. Maufroid;A. Capuron
通讯作者: J. Maufroid;A. Capuron
DOI: --
发表时间: 1985
期刊: Journal of embryology and experimental morphology
影响因子: --
作者:
P. Wigmore;N. Holder
通讯作者: N. Holder
由假定的外胚层和内胚层的组合形成组织者。
DOI: 10.2183/pjab1945.47.313
发表时间: 1971
期刊: The Journal of Cell Biology
影响因子: --
作者:
O. Nakamura;H. Takasaki;M. Ishihara
通讯作者: M. Ishihara
从卵到胚胎;J. M. W. Slack,第 241 页。(剑桥大学出版社,1983 年 8 月)25.00 英镑,49.50 美元。
DOI: 10.1113/expphysiol.1984.sp002828
发表时间: 1984
影响因子: 2.7
作者:
A. Warner
通讯作者: A. Warner
DOI: 10.1093/aesa/32.3.657
发表时间: 1938-10
期刊: The Yale Journal of Biology and Medicine
影响因子: --
作者:
Geo H Smith;R. R. Bensley-R.;L. Stone
通讯作者: Geo H Smith;R. R. Bensley-R.;L. Stone