Electromechanical and elastic probing of bacteria in a cell culture medium.
Electromechanical and elastic probing of bacteria in a cell culture medium.
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DOI：
10.1088/0957-4484/23/24/245705
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发表时间：
2012-06-22
期刊：
影响因子：
3.5
通讯作者：
Vertegel AA
中科院分区：
文献类型：
--
作者：
Thompson GL;Reukov VV;Nikiforov MP;Jesse S;Kalinin SV;Vertegel AA
培养细胞的快速表型表征和鉴定是组织工程和药物测试进步所需的，需要一种以亚微米分辨率测量细胞物理特性的实验技术。最近，带激发压电响应力显微镜（BEPFM）已被证明是有用的识别和成像的不同类型的细菌在纯水中。在此，BEPFM方法首次在生理学相关的电解质介质中进行，例如Dulbecco磷酸盐缓冲盐水（DPBS）和Dulbecco改良Eagle培养基（DMEM）。在DPBS中证明了溶壁微球菌（革兰氏阳性）和荧光假单胞菌（革兰氏阴性）细菌的不同机电响应。结果表明，每个细菌的外表面涂层的机械性能，以及它们周围的双电层，是负责在电解质介质中的BEPFM图像形成机制。
Rapid phenotype characterization and identification of cultured cells, which is needed for progress in tissue engineering and drug testing, requires an experimental technique that measures physical properties of cells with sub-micron resolution. Recently, band excitation piezoresponse force microscopy (BEPFM) has been proven useful for recognition and imaging of different types of bacteria in pure water. Here, the BEPFM method is performed for the first time in physiologically-relevant electrolyte media, such as Dulbecco’s phosphate-buffered saline (DPBS) and Dulbecco’s modified Eagle’s medium (DMEM). Distinct electromechanical responses for Micrococcus lysodeikticus (Gram-positive) and Pseudomonas fluorescens (Gram-negative) bacteria are demonstrated in DPBS. The results suggest that mechanical properties of the outer surface coating each bacterium, as well as the electrical double layer around them, are responsible for the BEPFM image formation mechanism in electrolyte media.
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影响因子：
4
作者：
Dokukin, M. E.;Guz, N. V.;Sokolov, I.
通讯作者：
Sokolov, I.
DOI：
10.1109/tei.1987.298955
发表时间：
1987-02-01
期刊：
IEEE TRANSACTIONS ON ELECTRICAL INSULATION
影响因子：
--
作者：
DASGUPTA, DK;DOUGHTY, K
通讯作者：
DOUGHTY, K
影响因子：
2.8
作者：
McGarry, James G.;Maguire, Paula;Jarvis, Suzanne P.
通讯作者：
Jarvis, Suzanne P.
影响因子：
3.2
作者：
Guo, S.;Ovchinnikov, O. S.;Kalinin, S. V.
通讯作者：
Kalinin, S. V.
影响因子：
3.5
作者：
Jesse, Stephen;Kalinin, Sergei V.
通讯作者：
Kalinin, Sergei V.