Identification of differentially expressed genes and pathways for intramuscular fat metabolism between breast and thigh tissues of chickens.
Identification of differentially expressed genes and pathways for intramuscular fat metabolism between breast and thigh tissues of chickens.
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鸡胸肌和大腿组织肌内脂肪代谢差异表达基因及通路鉴定
DOI：
10.1186/s12864-017-4292-3
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发表时间：
2018-01-16
期刊：
影响因子：
4.4
通讯作者：
Wen J
中科院分区：
文献类型：
--
作者：
Cui H;Zheng M;Zhao G;Liu R;Wen J
背景肌内脂肪（IMF）是影响鸡肉品质的重要因素之一，但其分子调控机制尚不清楚。本研究以北京油鸡42日龄和90日龄的胸肌和大腿肌内脂肪代谢为研究对象，系统地分析了肌内脂肪含量、基因本体论（GO）术语和京都基因与基因组百科全书（KEGG）途径的差异表达基因（DEG）及其分子调控机制。结果表明：42和90日龄时，乳房肌内脂肪含量显著低于大腿肌内脂肪含量（P< 0.05或P < 0.01）;通过基因芯片检测，在42和90日龄时，共检测到515个与肌内脂肪代谢相关的DEGs和36个与肌内脂肪代谢相关的DEGs。与大腿相比，乳腺中PPARG的表达水平显著下调（P< 0.01），而RXRA和CEBPB的表达水平显著上调（P< 0.01）。而LPL、FABP 4、THRSP、RBP 7、LDLR、FABP 3、CPT 2和PPARGC 1A在乳腺组织中的表达水平显著下调（P< 0.01），支持PPARG及其下游基因对IMF沉积具有重要的调控作用。此外，基于DEGs，KEGG分析揭示了PPAR信号通路和细胞连接相关通路（粘着斑和ECM-受体相互作用在维持组织完整性方面起着重要作用）可能参与鸡IMF代谢。提示鸡IMF代谢不仅受相关功能基因和PPAR通路的调控，还受细胞连接相关基因的调控。这些发现奠定了基础，并为破译家禽IMF沉积的分子机制提供了新的线索。现在需要在翻译和翻译后水平的进一步研究，以验证这里确定的基因和途径。
BackgroundIntramuscular fat (IMF) is one of the important factors influencing meat quality, however, for chickens, the molecular regulatory mechanisms underlying this trait have not yet been clear. In this study, a systematic identification of differentially expressed genes (DEGs) and molecular regulatory mechanism related to IMF metabolism between Beijing-you chicken breast and thigh at 42 and 90 days of age was performed.ResultsIMF contents, Gene Ontology (GO) terms, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were analyzed, The results showed that both IMF contents in breast at 42 and 90 d were significantly lower (P< 0.05 orP< 0.01) than those in thigh. By microarray, 515 common known DEGs and 36 DEGs related to IMF metabolism were identified between the breast and thigh at 42 and 90 d. Compared to thigh, the expression levels ofPPARGhad significantly down-regulated (P< 0.01) in breast, but the expression levels ofRXRAandCEBPBhad significantly up-regulated (P< 0.01). However, the expression levels ofLPL,FABP4,THRSP,RBP7,LDLR,FABP3,CPT2andPPARGC1Ahad significantly down-regulated in breast (P< 0.01), supporting thatPPARGand its down-stream genes had the important regulatory function to IMF deposition. In addition, based on of DEGs, KEGG analysis revealed that PPAR signaling pathway and cell junction-related pathways (focal adhesion and ECM-receptor interaction, which play a prominent role in maintaining the integrity of tissues), might contribute to the IMF metabolism in chicken.ConclusionsOur data had screened the potential candidate genes associated with chicken IMF metabolism, and imply that IMF metabolism in chicken is regulated and mediated not only by related functional genes and PPAR pathway, but also by others involved in cell junctions. These findings establish the groundwork and provide new clues for deciphering the molecular mechanisms underlying IMF deposition in poultry. Further studies at the translational and posttranslational level are now required to validate the genes and pathways identified here.
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影响因子：
64.5
作者：
Cao H;Gerhold K;Mayers JR;Wiest MM;Watkins SM;Hotamisligil GS
通讯作者：
Hotamisligil GS
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
56.9
作者：
BROWN, MS;GOLDSTEIN, JL
通讯作者：
GOLDSTEIN, JL
影响因子：
7.8
作者：
BORNSTEIN, P
通讯作者：
BORNSTEIN, P
DOI：
10.1097/00075197-200203000-00004
发表时间：
2002-03-01
影响因子：
3.1
作者：
Ibrahimi, A;Abumrad, NA
通讯作者：
Abumrad, NA