Development of microsatellite markers for the coastal shrub Scaevola taccada (Goodeniaceae).

Development of microsatellite markers for the coastal shrub Scaevola taccada (Goodeniaceae).
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DOI:
10.3732/apps.1300094
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发表时间:
2014-05
影响因子:
3.6
通讯作者:
Isagi Y
Isagi Y
中科院分区:
生物学4区
文献类型:
--
作者:
Ando H;Emura N;Denda T;Nakahama N;Inoue-Murayama M;Isagi Y

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·研究报告:利用微卫星标记技术,对滨海灌木种草菇的种群遗传结构进行了研究,以反映不同的种子扩散模式。方法和结果:共设计了13对微卫星引物。taccada使用454焦磷酸测序。在日本两个种群的64个个体上对引物组进行了测试。使用引物扩增片段,每个位点具有1至10个等位基因,预期杂合性范围为0.00至0.85。结论:这些结果表明标记物在S. taccada用于对该物种的种群遗传结构进行广泛的估计。
• Premise of the study: Microsatellite markers were developed for the coastal shrub species Scaevola taccada to estimate the population genetic structure, which may reflect different seed dispersal patterns. • Methods and Results: Thirteen microsatellite primer sets were developed for S. taccada using 454 pyrosequencing. The primer sets were tested on 64 individuals sampled from two populations in Japan. Fragments were amplified using the primers, with one to 10 alleles per locus, and the expected heterozygosity ranged from 0.00 to 0.85. • Conclusions: These results indicate the utility of markers in S. taccada for broad estimations of the population genetic structure of this species.
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