Functionalization of iron oxide magnetic nanoparticles with targeting ligands: their physicochemical properties and in vivo behavior.
Functionalization of iron oxide magnetic nanoparticles with targeting ligands: their physicochemical properties and in vivo behavior.
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DOI：
10.2217/nnm.10.55
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发表时间：
2010-11
期刊：
影响因子：
--
通讯作者：
Zhang M
中科院分区：
文献类型：
--
作者：
Fang C;Veiseh O;Kievit F;Bhattarai N;Wang F;Stephen Z;Li C;Lee D;Ellenbogen RG;Zhang M
通过分别靶向αvβ3整合素和MMP-2受体的精氨酸-甘氨酸-天冬氨酸（RGD）或氯毒素（CTX）配体对PEG固定的纳米颗粒进行功能化，开发和评价两种肿瘤特异性纳米探针。纳米探针由氧化铁核、生物相容性聚合物涂层和表面缀合的RGD或CTX肽制成。在体外和体内评估纳米探针的肿瘤靶向特异性。两种纳米探针都是高度分散的，并且在细胞培养基中表现出优异的长期稳定性。RGD-缀合的纳米探针在肿瘤中的新生血管附近显示出强烈的初始积累，随后快速清除。相反，CTX使能的纳米探针在整个肿瘤中表现出持续的积累。这些发现揭示了靶向配体对配体使能的纳米探针的肿瘤内分布的影响。通过灵活的表面化学，我们的纳米颗粒平台可以以模块化的方式用于结合生物分子以用于预期的应用。
To develop and evaluate two tumor-specific nanoprobes by functionalization of a PEG-immobilized nanoparticle with arginine-glycine-aspartic acid (RGD) or chlorotoxin (CTX) ligand that targets αvβ3 integrin and MMP-2 receptors, respectively. The nanoprobes were made of iron oxide cores, biocompatible polymer coating, and surface-conjugated RGD or CTX peptide. The tumor-targeting specificity of the nanoprobes was evaluated both in vitro and in vivo. Both nanoprobes were highly dispersive and exhibited excellent long-term stability in cell culture media. The RGD-conjugated nanoprobe displayed a strong initial accumulation near neovasculatures in tumors followed by quick clearance. Conversely, the CTX-enabled nanoprobe exhibited sustained accumulation throughout the tumor. These findings revealed the influence of the targeting ligands on the intratumoral distribution of the ligand-enabled nanoprobes. With flexible surface chemistry, our nanoparticle platform can be used in a modular fashion to conjugate biomolecules for intended applications.
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DOI：
10.1073/pnas.93.18.9764
发表时间：
1996-09-03
影响因子：
11.1
作者：
Friedlander, M;Theesfeld, CL;Cheresh, DA
通讯作者：
Cheresh, DA
影响因子：
4.8
作者：
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通讯作者：
Fayad, Zahi A.
影响因子：
56.9
作者：
BROOKS, PC;CLARK, RAF;CHERESH, DA
通讯作者：
CHERESH, DA
影响因子：
4.8
作者：
Montet, Xavier;Montet-Abou, Karin;Josephson, Lee
通讯作者：
Josephson, Lee
影响因子：
13.3
作者：
Fang, Chen;Bhattarai, Narayan;Sun, Conroy;Zhang, Miqin
通讯作者：
Zhang, Miqin