Functionalized nanoparticles with long-term stability in biological media.
Functionalized nanoparticles with long-term stability in biological media.
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DOI：
10.1002/smll.200801647
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发表时间：
2009-07
期刊：
影响因子：
13.3
通讯作者：
Zhang, Miqin
中科院分区：
文献类型：
--
作者：
Fang, Chen;Bhattarai, Narayan;Sun, Conroy;Zhang, Miqin
纳米粒子因其独特的光学、磁学或化学性质近年来得到了广泛的研究。[1-4]虽然已经研究了许多合成方法，但制备尺寸可控的超细单分散纳米粒子的有效方法是将前驱体在有机溶剂中高温热分解。[5，6]这种方法通过疏水涂层来稳定纳米粒子，因此合成的纳米粒子不能分散在水溶液中。在生物医学应用中，纳米颗粒必须是亲水性的，并在生物介质中保持优异的稳定性。对于先进的纳米粒子的生物医学应用(例如，体内诊断和治疗)，必须施加额外的要求，例如最小化网状内皮系统(RES)的非特异性摄取，以实现长血液循环时间和高诊断或治疗效率。此外，纳米粒子的表面应该具有进一步结合靶向配体或治疗剂的官能团。常用的亲水聚合物包覆疏水纳米粒子的方法有：配体交换法、胶束包覆法、[9]和共价键。尤其是亲水性聚乙二醇组分，由于其具有抗蛋白质污染和空间位阻防止纳米粒子团聚的能力，已成为纳米粒子[2，12-15]的有效包覆材料。17]典型的例子包括通过配体交换将聚乙二醇包覆在疏水纳米粒子上，其中多巴胺连接的聚乙二醇取代粒子上的油胺和油酸[18]，以及在水共沉淀过程中通过共价键原位将聚乙二醇包覆在氧化铁纳米粒子上。[10]尽管这些方法取得了进展，但在纳米粒子上产生高度稳定的聚合物涂层并保持功能化纳米粒子在生物相关介质中的长期稳定性仍然是一个挑战。在这里，我们提出了一种强大的表面工程方法来制备超细、单分散、亲水性和功能化的氧化物纳米粒子，这些纳米粒子在生物介质中表现出长期的胶体稳定性和低的非特异性巨噬细胞摄取。我们以纳米氧化铁为模型系统演示了这一方法。由于其超顺磁性和良好的生物相容性，氧化铁纳米颗粒已被广泛研究用于生物医学应用，包括磁共振成像(MRI)、细胞标记和跟踪以及靶向治疗输送。
Nanoparticles have been extensively studied in recent years due to their unique optical, magnetic, or chemical properties.[1-4] While many synthetic methods have been investigated, an effective way to produce ultrafine and monodisperse nanoparticles with controllable sizes is thermal decomposition of precursors in organic solvents at high temperature.[5, 6] In this approach, nanoparticles are stabilized by hydrophobic coatings and as a result, the assynthesized nanoparticles can not be dispersed in aqueous solutions. In biomedical applications, nanoparticles have to be hydrophilic and maintain a superior stability in biological media. For advanced biomedical applications of nanoparticles (eg, in vivo diagnostics and therapy), additional requirements such as minimization of non-specific uptake by reticuloendothelial systems (RES) must be imposed in order to achieve long blood circulation time and high diagnostic or therapeutic efficiency.[7] In addition, the surface of the nanoparticle should possess functional groups for further conjugation of targeting ligand or therapeutic agents. Commonly used modification strategies for coating hydrophobic nanoparticles with hydrophilic polymers include ligand exchange,[8] micelle encapsulation,[9] and covalent bonding.[10][11] Particularly, hydrophilic poly (ethylene glycol)(PEG) have been the focus of research as an effective coating materials for nanoparticles [2, 12-15] due to its ability to resist protein fouling and provide steric hindrance preventing nanoparticle from aggregation.[12, 16, 17] Typical examples include coating PEG on hydrophobic nanoparticles via ligand exchange in which dopamine linked PEG replaces oleylamine & oleic acid on the particle [18] and coating PEG on iron oxide nanoparticles in situ via covalent bonding during the aqueous co-precipitation process.[10] Despite the advances made with those methods, the challenge remains in producing a highly stable polymeric coating on nanoparticles and retaining the long-term stability of functionalized nanoparticles in biological-relevant media.Here, we present a robust surface engineering approach to produce ultrafine, monodisperse, hydrophilic and functionalized oxide nanoparticles that display long-term colloidal stability in biological media and low non-specific uptake by macrophage cells. We demonstrated this approach with iron oxide nanoparticles as our model system. Due to their superparamagnetic properties and excellent biocompatibility, iron oxide nanoparticles have been extensively studied for biomedical applications including magnetic resonance imaging (MRI), cell labeling and tracking, and targeted therapeutic delivery.[1, 3, 19]
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影响因子：
15
作者：
Kohler, N;Fryxell, GE;Zhang, MQ
通讯作者：
Zhang, MQ
影响因子：
15
作者：
Yu, William W.;Chang, Emmanuel;Colvin, Vicki L.
通讯作者：
Colvin, Vicki L.
影响因子：
29.4
作者：
Xie, Jin;Xu, Chenjie;Sun, Shouheng
通讯作者：
Sun, Shouheng
影响因子：
41.2
作者：
Park, J;An, KJ;Hyeon, T
通讯作者：
Hyeon, T
DOI：
10.1016/j.ejpb.2007.02.024
发表时间：
2008-01-01
影响因子：
4.9
作者：
Petri-Fink, Alke;Steitz, Benedikt;Hofmann, Heinrich
通讯作者：
Hofmann, Heinrich