Proteinase-activated receptor-2 up-regulation by Fcgamma-receptor activation in human neutrophils.
Proteinase-activated receptor-2 up-regulation by Fcgamma-receptor activation in human neutrophils.
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DOI：
10.1096/fj.09-146167
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发表时间：
2010-06
期刊：
影响因子：
--
通讯作者：
Pouliot M
中科院分区：
文献类型：
--
作者：
St-Onge M;Lagarde S;Laflamme C;Rollet-Labelle E;Marois L;Naccache PH;Pouliot M
我们揭示了与炎症和痛觉过敏相关的蛋白酶激活受体（PAR）家族在人粒细胞中的表达和功能。静息细胞表达组成水平的PAR-2和PAR-3 mRNA，但不表达PAR-1或PAR-4。基于流式细胞术，调理菌（opsonized bacteria, Bop）刺激以浓度依赖和时间依赖的方式特异性上调细胞表面PAR-2的表达，独立于转录或从头蛋白合成。原生颗粒被确定为预制PAR-2的来源，可以很容易地在与质膜融合时在表面动员。通过测量细胞内钙浓度的变化，细胞对PAR-2激活的反应在PAR-2上调的细胞中增强。细胞表面PAR-2和细胞反应性的增加特异性地依赖于免疫球蛋白（Ig）结合受体的参与。总之，我们的研究结果表明，细胞内颗粒的动员响应于ig受体的激活，上调PAR-2表面表达，并使中性粒细胞对蛋白酶活性更敏感。这种对PAR-2激活的增强反应表明，疼痛和炎症之间的分子交流可能比以前认为的更重要。
We shed new light on the expression and function of the proteinase-activated receptor (PAR) family, associated with inflammation and hyperalgesia, in human granulocytes. Resting cells expressed constitutive levels of PAR-2 and PAR-3 mRNA but not PAR-1 or PAR-4. Based on flow cytometry, stimulation with opsonized bacteria (Bop) specifically up-regulated cell surface expression of PAR-2 in a concentration-dependent and time-dependent manner, independent of transcription or de novo protein synthesis. Primary granules were identified as a source of preformed PAR-2 that can readily be mobilized at the surface on fusion with the plasma membrane. Cellular response to PAR-2 activation, measured as changes in intracellular calcium concentration, was enhanced in PAR-2 up-regulated cells. Increase of cell-surface PAR-2 and of cell responsiveness were dependent specifically on the engagement of immunoglobulin (Ig)-binding receptors. Together, our results reveal that mobilization of intracellular granules, in response to Ig-receptor activation, up-regulates PAR-2 surface expression and makes neutrophils more responsive to proteinase activity. This enhanced response to PAR-2 activation indicates that molecular communication between pain and inflammation may be more important than previously believed.
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DOI：
10.4049/jimmunol.181.8.5702
发表时间：
2008-10-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Chin AC;Lee WY;Nusrat A;Vergnolle N;Parkos CA
通讯作者：
Parkos CA
DOI：
10.1165/rcmb.2004-0274oc
发表时间：
2005-05-01
影响因子：
6.4
作者：
Dulon, S;Leduc, D;Chignard, M
通讯作者：
Chignard, M
影响因子：
2.5
作者：
Foster PS;Rosenberg HF;Asquith KL;Kumar RK
通讯作者：
Kumar RK
影响因子：
5.9
作者：
LYNCH, JP;FLINT, A;FANTONE, JC
通讯作者：
FANTONE, JC
影响因子：
64.8
作者：
Coughlin, SR
通讯作者：
Coughlin, SR