Intracellular forces during guided cell growth on micropatterns using FRET measurement.
Intracellular forces during guided cell growth on micropatterns using FRET measurement.
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DOI：
10.1016/j.jbiomech.2014.12.051
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发表时间：
2015-02-26
影响因子：
2.4
通讯作者：
Hua, Susan Z.
中科院分区：
文献类型：
--
作者：
Suffoletto, Kevin;Ye, Nannan;Meng, Fanjie;Verma, Deepika;Hua, Susan Z.
细胞与细胞外基质（ECM）的相互作用调节着细胞的形状、分化和极性。这种效应在具有各种图案特征、硬度和表面化学性质的基质上生长的细胞中被广泛观察到。据推测，底物对细胞的机械限制会导致细胞骨架蛋白张力的重新分配，从而通过力敏感途径导致细胞骨架重组。然而，重组过程中的力传导机制仍不清楚。在这项研究中，我们使用基于FRET的力传感器测量了肌动蛋白交联蛋白α-肌动蛋白的张力，并跟踪了在图案底物上生长的HEK细胞中肌动蛋白细胞骨架的实时重组。我们发现，有图案的底物引起α-肌动蛋白中张力的重新分配，这与细胞骨架重组相一致。在部分细胞中观察到较高的张力，在这些细胞中，它们在图案底物的抑制区域形成桥；发现附着在基材上可以释放张力。α-肌动蛋白张力和f -肌动蛋白排列的实时测量结果表明，当细胞在抑制区扩散时，张力的增加与细胞周围f -肌动蛋白束的形成一致，这表明机械力刺激了细胞骨架的增强。Rho-ROCK抑制剂（Y27632）导致肌动蛋白张力降低，随后桥接区域收缩。我们的研究结果表明，细胞形状的变化和在图案表面上的扩张是一个力敏感的过程，需要肌动球蛋白收缩力参与Rho-ROCK途径。
Interaction of cells with extracellular matrix (ECM) regulates cell shape, differentiation and polarity. This effect has been widely observed in cells grown on substrates with various patterned features, stiffness and surface chemistry. It has been postulated that mechanical confinement of cells by the substrate causes a redistribution of tension in cytoskeletal proteins resulting in cytoskeletal reorganization through force sensitive pathways. However, the mechanisms for force transduction during reorganization remain unclear. In this study, using FRET based force sensors we have measured tension in an actin cross-linking protein, α-actinin, and followed reorganization of actin cytoskeleton in real time in HEK cells grown on patterned substrates. We show that the patterned substrates cause a redistribution of tension in α-actinin that coincides with cytoskeleton reorganization. Higher tension was observed in portions of cells where they form bridges across inhibited regions of the patterned substrates; the attachment to the substrate is found to release tension. Real time measurements of α-actinin tension and F-actin arrangement show that an increase in tension coincides with formation of F-actin bundles at the cell periphery during cell-spreading across inhibited regions, suggesting that mechanical forces stimulate cytoskeleton enhancement. Rho-ROCK inhibitor (Y27632) causes reduction in actinin tension followed by retraction of bridged regions. Our results demonstrate that changes in cell shape and expansion over patterned surfaces is a force sensitive process that requires actomyosin contractile force involving Rho-ROCK pathway.
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影响因子：
9.7
作者：
Polio, Samuel R.;Rothenberg, Katheryn E.;Stamenovic, Dimitrije;Smith, Michael L.
通讯作者：
Smith, Michael L.
DOI：
10.1038/nrm3112
发表时间：
2011-05
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.4
作者：
Sabass, Benedikt;Gardel, Margaret L.;Schwarz, Ulrich S.
通讯作者：
Schwarz, Ulrich S.
DOI：
10.1016/s0928-4931(02)00309-0
发表时间：
2003-03-03
期刊：
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
影响因子：
--
作者：
Schwarz, US;Balaban, NQ;Geiger, B
通讯作者：
Geiger, B
影响因子：
--
作者：
Karuri, Nancy W.;Nealey, Paul F.;Murphy, Christopher J.;Albrecht, Ralph M.
通讯作者：
Albrecht, Ralph M.