Morphological and functional characteristics of human gingival junctional epithelium.
Morphological and functional characteristics of human gingival junctional epithelium.
复制标题
DOI：
10.1186/1472-6831-14-30
复制
发表时间：
2014-04-03
期刊：
影响因子：
2.9
通讯作者：
Guo X
中科院分区：
文献类型：
--
作者：
Jiang Q;Yu Y;Ruan H;Luo Y;Guo X
本研究旨在观察日本血吸虫病（JE）组织和培养的JE细胞的形态学特征，并鉴定其功能特性。对6名受试者颊舌侧的磨牙或前磨牙进行石蜡切片。采用HE染色和图像分析技术，比较JE、口腔牙龈上皮（OGE）和龈沟上皮（SE）的形态学差异。应用免疫组化法检测细胞角蛋白5/6、7、8/18、10/13、16、17、19、20在乙脑、OGE、SE中的表达情况。另一方面，体外培养原代人JE和OGE细胞。通过组织学和免疫组化证实细胞的鉴定。在共培养模型中，透射电镜观察JE细胞与牙面的粘附情况。人JE是一种形态上不同于SE和OGE的独特组织。与体外培养的OGE细胞相比，JE细胞的形态也有其特殊性。此外，JE细胞比OGE细胞具有更长的潜伏期。几种CK的表达差异说明乙脑细胞具有低分化、高再生的特点。共培养14 d后，JE细胞膜与牙面交界处出现多层细胞、基底膜样和半桥粒样结构。JE是一种特殊的复层上皮，在体内和体外牙龈组织中具有低分化和高再生能力。在共培养模型中，人JE细胞可在2周左右形成基底膜样和半桥粒样结构。
This study aims to observe the morphological characteristics and identify the function characteristics of junctional epithelium (JE) tissues and cultured JE cells. Paraffin sections of human molar or premolar on the gingival buccolingual side were prepared from 6 subjects. HE staining and image analysis were performed to measure and compare the morphological difference among JE, oral gingival epithelium (OGE) and sulcular epithelium (SE). Immunohistochemistry was applied to detect the expression pattern of cytokeratin 5/6, 7, 8/18, 10/13, 16, 17, 19, and 20 in JE, OGE and SE. On the other hand, primary human JE and OGE cells were cultured in vitro. Cell identify was confirmed by histology and immunohistochemistry. In a co-culture model, TEM was used to observe the attachment formation between JE cells and tooth surface. Human JE was a unique tissue which was different from SE and OGE in morphology. Similarly, morphology of JE cells was also particular compared with OGE cells cultured in vitro. In addition, JE cells had a longer incubation period than OGE cells. Different expression of several CKs illustrated JE was in a characteristic of low differentiation and high regeneration. After being co-cultured for 14 d, multiple cell layers, basement membrane-like and hemidesmosome-like structures were appeared at the junction of JE cell membrane and tooth surface. JE is a specially stratified epithelium with low differentiation and high regeneration ability in gingival tissue both in vivo and in vitro. In co-culture model, human JE cells can form basement membrane-like and hemidesmosome-like structures in about 2 weeks.
登录
查看更多内容
影响因子：
4.3
作者：
Papaioannou, W;Cassiman, JJ;Quirynen, M
通讯作者：
Quirynen, M
人角蛋白：生物学和病理学。
影响因子：
2.3
作者：
Moll, Roland;Divo, Markus;Langbein, Lutz
通讯作者：
Langbein, Lutz
影响因子：
3
作者：
Barrett, AW;Cort, EM;Berkovitz, BKB
通讯作者：
Berkovitz, BKB
影响因子：
4.3
作者：
Gursoy, Ulvi K.;Kononen, Eija;Uitto, Veli-Jukka
通讯作者：
Uitto, Veli-Jukka
影响因子：
3.5
作者：
ABE, Y;HARA, Y;KATO, I
通讯作者：
KATO, I