Myelin‐Associated Oligodendrocytic Basic Protein mRNAs Reside at Different Subcellular Locations
Myelin‐Associated Oligodendrocytic Basic Protein mRNAs Reside at Different Subcellular Locations
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髓磷脂相关少突胶质细胞碱性蛋白 mRNA 位于不同的亚细胞位置
DOI：
10.1046/j.1471-4159.1999.01913.x
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发表时间：
1999
影响因子：
4.7
通讯作者：
Elisa Barbarese
中科院分区：
文献类型：
--
作者：
Robert M. Gould;C. Freund;Elisa Barbarese
髓磷脂碱性蛋白 (MBP) 和髓磷脂相关少突胶质细胞碱性蛋白 (MOBP)-81A 两种髓磷脂蛋白的 mRNA 独特地位于髓鞘组装的位点。在这里，我们使用亚细胞分离来显示四个 MOBP mRNA（如 MBP mRNA）位于髓鞘组装位点，而其他三个 MOBP mRNA 位于少突胶质细胞体中。 MOBP-81 蛋白存在于髓磷脂和另一个亚细胞部分中，而其他髓磷脂蛋白，包括 MBP、29,39-环核苷酸 39-磷酸二酯酶和髓磷脂相关糖蛋白，很大程度上仅限于髓磷脂。通过选择性剪接产生不同的 MBP mRNA。它们都包含一个 RNA 转运序列 (RTS)，将它们引导至少突胶质细胞中组装髓鞘的位点。因此，所有这些都富含髓磷脂。分级分离后，四种 MOBP mRNA：MOBP71、MOBP-81A、MOBP-99 和 MOBP-169（在本研究中鉴定）在髓磷脂中富集。这些 mRNA 包含一个共同的外显子，即外显子 8b，其核苷酸序列与 MBP mRNA RTS 相似。该序列可能将这些 mRNA 引导至髓鞘组装位点。其他三种 MOBP mRNA：MOBP-69、MOBP-81B 和 MOBP-170 缺乏该外显子。它们的亚细胞分布表明它们大部分保留在少突胶质细胞体中。我们得出结论，少突胶质细胞中 MOBP 的分布受到相应 mRNA 选择性剪接的强烈影响。
The mRNAs for two myelin proteins, myelin basic protein (MBP) and myelin-associated oligodendrocytic basic protein (MOBP)-81A, are uniquely located at sites where myelin sheaths are assembled. Here, we use subcellular fractionation to show that four MOBP mRNAs, like MBP mRNA, are located at sites of myelin sheath assembly, and that three other MOBP mRNAs are located in oligodendrocyte soma. The MOBP-81 protein is found in myelin and in another subcellular fraction, whereas other myelin proteins, including MBP, 29,39-cyclic nucleotide 39-phosphodiesterase, and myelin-associated glycoprotein, are largely restricted to myelin. Different MBP mRNAs are generated by alternative splicing. All of them contain an RNA transport sequence (RTS) that directs them to sites in oligodendrocytes, where myelin sheaths are assembled. Consequently, all are enriched in myelin. After fractionation, four MOBP mRNAs, MOBP71, MOBP-81A, MOBP-99, and MOBP-169 (identified in this study), are enriched in myelin. These mRNAs contain a common exon, exon 8b, which has a nucleotide sequence that is similar to MBP mRNA RTS. This sequence likely directs these mRNAs to sites of myelin sheath assembly. Three other MOBP mRNAs, MOBP-69, MOBP-81B, and MOBP-170, lack this exon. Their subcellular distribution indicates that they are largely retained in oligodendrocyte soma. We conclude that the distribution of MOBPs in oligodendrocytes is strongly influenced by alternative splicing of the corresponding mRNAs.
DOI：
10.1016/s0021-9258(18)53485-2
发表时间：
1993-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
A. Campagnoni;T. Pribyl;C. Campagnoni;K. Kampf;S. Amur‐Umarjee;C. Landry;V. Handley;S. Newman;B. Garbay;K. Kitamura
通讯作者：
A. Campagnoni;T. Pribyl;C. Campagnoni;K. Kampf;S. Amur‐Umarjee;C. Landry;V. Handley;S. Newman;B. Garbay;K. Kitamura
编码髓磷脂特异性蛋白的 mRNA 的空间分离。
DOI：
10.1073/pnas.84.21.7773
发表时间：
1987
影响因子：
11.1
作者：
Trapp,BD;Moench,T;Pulley,M;Barbosa,E;Tennekoon,G;Griffin,J
通讯作者：
Griffin,J