Developmental profiling of gene expression in soybean trifoliate leaves and cotyledons.
Developmental profiling of gene expression in soybean trifoliate leaves and cotyledons.
复制标题
DOI：
10.1186/s12870-015-0553-y
复制
发表时间：
2015-07-03
影响因子：
5.3
通讯作者：
Hudson KA
中科院分区：
文献类型：
--
作者：
Brown AV;Hudson KA
发芽后，正在发育的大豆幼苗依靠子叶中储存的营养储备来维持异养生长。在种子灌浆期，发育中的种子依靠三叶叶输送养分。在大豆中，子叶和叶子都发展出光合作用的能力，一旦它们的功能结束，就会衰老和脱落。在此之前，它们所含的营养物质被动员并运输到植物的其他部分。这些过程是在叶子或子叶的整个发育过程中通过遗传调控精心协调的。为了鉴定大豆叶片或子叶发育和衰老过程中涉及的基因，我们使用 RNA-seq 来分析子叶和叶片组织的多个阶段。确定了叶或子叶发育阶段之间差异表达的基因，定义了基因表达的主要模式，并鉴定了共有基因。在叶和子叶发育过程中表达了超过 38,000 个转录本。在这些转录本中，有 5,000 个以组织特异性模式表达。在两个后期组织之间差异表达的基因中，90% 具有相同的变化方向，这表明衰老机制在组织之间是保守的。对共享共同表达谱的基因内生物功能富集的分析突出了在叶和子叶发育的这些定义的时间窗口内发生的主要过程。超过 1,000 个基因被鉴定出具有预测的调节功能，这些功能可能在控制叶子或子叶衰老中发挥作用。叶子和子叶的发育过程可以分为以特定基因组的表达为特征的不同阶段。 WRKY、NAC 和 GRAS 家族转录因子作为植物衰老主要调节因子的重要性在大豆叶和子叶组织中得到了证实。这些结果有助于验证大豆基因和启动子的功能注释。本文的在线版本 (doi:10.1186/s12870-015-0553-y) 包含补充材料，可供授权用户使用。
Immediately following germination, the developing soybean seedling relies on the nutrient reserves stored in the cotyledons to sustain heterotrophic growth. During the seed filling period, developing seeds rely on the transport of nutrients from the trifoliate leaves. In soybean, both cotyledons and leaves develop the capacity for photosynthesis, and subsequently senesce and abscise once their function has ended. Before this occurs, the nutrients they contain are mobilized and transported to other parts of the plant. These processes are carefully orchestrated by genetic regulation throughout the development of the leaf or cotyledon. To identify genes involved in the processes of leaf or cotyledon development and senescence in soybean, we used RNA-seq to profile multiple stages of cotyledon and leaf tissues. Differentially expressed genes between stages of leaf or cotyledon development were determined, major patterns of gene expression were defined, and shared genes were identified. Over 38,000 transcripts were expressed during the course of leaf and cotyledon development. Of those transcripts, 5,000 were expressed in a tissue specific pattern. Of the genes that were differentially expressed between both later stage tissues, 90 % had the same direction of change, suggesting that the mechanisms of senescence are conserved between tissues. Analysis of the enrichment of biological functions within genes sharing common expression profiles highlights the main processes occurring within these defined temporal windows of leaf and cotyledon development. Over 1,000 genes were identified with predicted regulatory functions that may have a role in control of leaf or cotyledon senescence. The process of leaf and cotyledon development can be divided into distinct stages characterized by the expression of specific gene sets. The importance of the WRKY, NAC, and GRAS family transcription factors as major regulators of plant senescence is confirmed for both soybean leaf and cotyledon tissues. These results help validate functional annotation for soybean genes and promoters. The online version of this article (doi:10.1186/s12870-015-0553-y) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
7.4
作者：
Gan, SS;Amasino, RM
通讯作者：
Amasino, RM
影响因子：
13.8
作者：
Gregersen, Per L.;Holm, Preben Bach
通讯作者：
Holm, Preben Bach
DOI：
10.1111/tpj.12194
发表时间：
2013-07
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Hickman R;Hill C;Penfold CA;Breeze E;Bowden L;Moore JD;Zhang P;Jackson A;Cooke E;Bewicke-Copley F;Mead A;Beynon J;Wild DL;Denby KJ;Ott S;Buchanan-Wollaston V
通讯作者：
Buchanan-Wollaston V
DOI：
10.1073/pnas.85.19.7089
发表时间：
1988-10-01
影响因子：
11.1
作者：
GIULIANO, G;PICHERSKY, E;CASHMORE, AR
通讯作者：
CASHMORE, AR
影响因子：
9.4
作者：
HARRIS, M;MACKENDER, RO;SMITH, DL
通讯作者：
SMITH, DL