The Hypervariable Tpr Multigene Family of Theileria Parasites, Defined by a Conserved, Membrane-Associated, C-Terminal Domain, Includes Several Copies with Defined Orthology Between Species.
The Hypervariable Tpr Multigene Family of Theileria Parasites, Defined by a Conserved, Membrane-Associated, C-Terminal Domain, Includes Several Copies with Defined Orthology Between Species.
复制标题
DOI：
10.1007/s00239-023-10142-z
复制
发表时间：
2023-12
影响因子：
3.9
通讯作者：
中科院分区：
文献类型：
--
作者：
多基因家族在宿主-寄生虫相互作用中起着重要作用。东海岸热的病原体微小泰勒虫中最大的多基因家族之一是微小泰勒虫重复(Tpr)基因家族。推测的Tpr蛋白的功能仍不清楚。最初发表的微小毛滴虫参考基因组确定了39个Tpr家族开放阅读框架(ORF)，它们共享一个保守的C-末端结构域。其中28个基因聚集在3号染色体的中心区域，称为“Tpr基因座”，而其他基因则分布在所有四条核染色体上。Tpr基因座包含基因组中剩余的四个组装间隙中的三个，这表明存在额外的、尚未确定的Tpr基因副本。在这里，我们描述了利用长读测序的方法来试图填补微小毛滴虫(位于多基因家族簇之间)参考组装中的空白，描述微小毛滴虫参考基因组中Tpr家族ORF的完整补充，并评估它们与其他泰勒虫Tpr同源物的进化关系。我们在微小毛滴虫参考基因组中发现了三个新的Tpr家族基因，并表明Tpr基因座上的Paralog之间的序列相似性显着高于Tpr基因座外的基因之间的序列相似性。我们还在另外五种泰勒虫中鉴定了与保守的C-末端结构域同源的序列。利用这些序列，我们证明了这个基因家族的进化涉及到跨物种的少数同源基因的保守，结合基因的得失，以及物种特有的扩展。网上版载有补充材料，可在10.1007/s00239-023-10142-z查阅。
Multigene families often play an important role in host-parasite interactions. One of the largest multigene families in Theileria parva, the causative agent of East Coast fever, is the T. parva repeat (Tpr) gene family. The function of the putative Tpr proteins remains unknown. The initial publication of the T. parva reference genome identified 39 Tpr family open reading frames (ORFs) sharing a conserved C-terminal domain. Twenty-eight of these are clustered in a central region of chromosome 3, termed the “Tpr locus”, while others are dispersed throughout all four nuclear chromosomes. The Tpr locus contains three of the four assembly gaps remaining in the genome, suggesting the presence of additional, as yet uncharacterized, Tpr gene copies. Here, we describe the use of long-read sequencing to attempt to close the gaps in the reference assembly of T. parva (located among multigene families clusters), characterize the full complement of Tpr family ORFs in the T. parva reference genome, and evaluate their evolutionary relationship with Tpr homologs in other Theileria species. We identify three new Tpr family genes in the T. parva reference genome and show that sequence similarity among paralogs in the Tpr locus is significantly higher than between genes outside the Tpr locus. We also identify sequences homologous to the conserved C-terminal domain in five additional Theileria species. Using these sequences, we show that the evolution of this gene family involves conservation of a few orthologs across species, combined with gene gains/losses, and species-specific expansions. The online version contains supplementary material available at 10.1007/s00239-023-10142-z.
登录
查看更多内容
影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
使用MPSS对原生动物Theileria parva的转录组分析表明，大多数基因在Schizont阶段具有转录活性。
影响因子：
14.9
作者：
Bishop, R;Shah, T;Pelle, R;Hoyle, D;Pearson, T;Haines, L;Brass, A;Hulme, H;Graham, SP;Taracha, ELN;Kanga, S;Lu, C;Hass, B;Wortman, J;White, O;Gardner, MJ;Nene, V;de Villiers, EP
通讯作者：
de Villiers, EP
影响因子：
5.2
作者：
Gomes PS;Bhardwaj J;Rivera-Correa J;Freire-De-Lima CG;Morrot A
通讯作者：
Morrot A
影响因子：
30.8
作者：
Chakraborty M;VanKuren NW;Zhao R;Zhang X;Kalsow S;Emerson JJ
通讯作者：
Emerson JJ
影响因子：
10.7
作者：
Darriba, Diego;Posada, David;Flouri, Tomas
通讯作者：
Flouri, Tomas