Combination of plasma HA and circulating M2-like monocytes may serve as a diagnostic marker for breast cancer.
Combination of plasma HA and circulating M2-like monocytes may serve as a diagnostic marker for breast cancer.
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血浆 HA 和循环 M2 样单核细胞的组合可作为乳腺癌的诊断标志物
DOI：
10.7150/jca.20227
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发表时间：
2017
影响因子：
3.9
通讯作者：
Gao F
中科院分区：
文献类型：
--
作者：
Zhang B;Cao M;He Y;Liu Y;Zhang G;Yang C;Du Y;Xu J;Hu J;Gao F
背景：乳腺癌(BC)来源的透明质酸(HA)可诱导肿瘤组织中M2样肿瘤相关巨噬细胞(TAMs)的形成。然而，BC患者外周血中类M2单核细胞与血浆HA之间的相关性却鲜为人知。本研究旨在探讨外周血中类M2单核细胞与血浆透明质酸之间的关系，并进一步评价其在BC中的诊断价值。方法：采用免疫组织化学方法检测病理组织中M2样TAMs和HA的表达。应用流式细胞仪检测81例乳腺癌患者、45例乳腺良性疾病患者和46例健康体检者外周血中CD14+CD204+M2样单核细胞水平。用化学发光法测定血浆中HA、CEA、CA15-3水平。结果：结直肠癌组织中M2样TAMs和HA的表达均高于良性组织。相应地，BC患者外周血中CD14+CD204+M2样单核细胞比例和血浆HA水平明显高于对照组。循环中M2样单核细胞与血浆透明质酸呈正相关(Spearman r=0.404，p<；0.001)。循环M2样单核细胞联合透明质酸组受者工作特征曲线下面积为0.899(95%CI：0.853~0.946)，高于癌胚抗原组和CA15-3组。结论：CD14+CD204+M2样单核细胞比例与血浆HA水平呈正相关。联合检测外周血CD14+CD204+M2样单核细胞和血浆透明质酸对BC具有较高的诊断价值。
Background: Breast cancer (BC)-derived hyaluronan (HA) can induce the formation of M2-like tumor-associated macrophages (TAMs) in tumor context. However, little is known about the correlation between circulating M2-like monocytes and plasma HA in BC patients. This study focused on evaluating the relationship between circulating M2-like monocytes and plasma HA, and further appraised the diagnostic value of them in BC. Methods: The expression of M2-like TAMs and HA was determined in pathological tissues by immunohistochemistry. Flow cytometry was used to detect the levels of circulating CD14+CD204+ M2-like monocytes in 81 BC patients, 45 patients with breast benign diseases, and 46 healthy subjects. The levels of HA, CEA, and CA15-3 were measured in plasma samples using chemiluminescence method. Results: M2-like TAMs and HA expressions were elevated in BC tissues compared with benign tissues. In correspondence, the frequency of circulating CD14+CD204+ M2-like monocytes and the plasma HA levels were significantly higher in patients with BC than those in control groups. Importantly, there was a positive correlation between circulating M2-like monocytes and the plasma HA (Spearman r = 0.404, p < 0.001). Area under receiver operating characteristic curve (ROC) for the combination of circulating M2-like monocytes and HA was 0.899 (95% CI: 0.853-0.946), which was higher than the panel of CEA and CA15-3. Conclusions: The frequency of circulating CD14+CD204+ M2-like monocytes was positively correlated to plasma HA levels. The combination of circulating CD14+CD204+ M2-like monocytes and plasma HA could provide considerable diagnostic value in BC.
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影响因子：
8.8
作者：
Schmaus, A.;Klusmeier, S.;Rothley, M.;Dimmler, A.;Sipos, B.;Faller, G.;Thiele, W.;Allgayer, H.;Hohenberger, P.;Post, S.;Sleeman, J. P.
通讯作者：
Sleeman, J. P.
DOI：
10.1073/pnas.1320198111
发表时间：
2014-03-04
影响因子：
11.1
作者：
Adams, Daniel L.;Martin, Stuart S.;Cristofanilli, Massimo
通讯作者：
Cristofanilli, Massimo
影响因子：
254.7
作者：
DeSantis, Carol;Ma, Jiemin;Jemal, Ahmedin
通讯作者：
Jemal, Ahmedin
影响因子：
7.3
作者：
Chávez-Galán L;Olleros ML;Vesin D;Garcia I
通讯作者：
Garcia I
影响因子：
45.3
作者：
Harvey, JM;Clark, GM;Allred, DC
通讯作者：
Allred, DC