Chlamydomonas IFT70/CrDYF-1 is a core component of IFT particle complex B and is required for flagellar assembly.
Chlamydomonas IFT70/CrDYF-1 is a core component of IFT particle complex B and is required for flagellar assembly.
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DOI：
10.1091/mbc.e10-03-0191
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发表时间：
2010-08-01
影响因子：
3.3
通讯作者：
Qin H
中科院分区：
文献类型：
--
作者：
Fan ZC;Behal RH;Geimer S;Wang Z;Williamson SM;Zhang H;Cole DG;Qin H
DYF-1是一个高度保守的蛋白质。我们的研究结果表明，DYF-1是一个典型的亚基IFT颗粒复合物B，并强烈支持这一假设，即IFT机器具有物种和组织特异性的变化与功能的分歧。DYF-1是一种高度保守的蛋白质，在几种模式生物中对纤毛发生至关重要。在秀丽隐杆线虫中，DYF-1作为鞭毛内运输（IFT）的顺行马达OSM-3的必需激活剂，纤毛发生所需的运动介导鞭毛前体的运输和周转产物的去除。在斑马鱼和四膜虫中，DYF-1影响纤毛微管蛋白的翻译后修饰，并且可能比OSM-3在纤毛发生中具有更普遍的功能。在这里，我们解决了DYF-1生物化学相互作用的IFT机械通过使用模式生物莱茵衣藻，其中顺行IFT不依赖于OSM-3。我们的研究结果表明，这种蛋白质是IFT颗粒复合物B的化学计量组分，并直接与复合物B亚基IFT 46相互作用。与已建立的IFT蛋白命名法一致，DYF-1也以蛋白的表观大小命名为IFT 70。IFT在构建鞭毛中的功能是必需的，因为去除了IFT 70/CrDYF-1的细胞的鞭毛大大缩短。总之，这些结果表明，IFT 70/CrDYF-1是IFT颗粒复合物B的典型亚基，并强烈支持IFT机制具有物种和组织特异性变异的功能分支的假设。
DYF-1 is a highly conserved protein. Our results demonstrate that DYF-1 is a canonical subunit of IFT particle complex B and strongly support the hypothesis that the IFT machinery has species- and tissue-specific variations with functional ramifications. DYF-1 is a highly conserved protein essential for ciliogenesis in several model organisms. In Caenorhabditis elegans, DYF-1 serves as an essential activator for an anterograde motor OSM-3 of intraflagellar transport (IFT), the ciliogenesis-required motility that mediates the transport of flagellar precursors and removal of turnover products. In zebrafish and Tetrahymena DYF-1 influences the cilia tubulin posttranslational modification and may have more ubiquitous function in ciliogenesis than OSM-3. Here we address how DYF-1 biochemically interacts with the IFT machinery by using the model organism Chlamydomonas reinhardtii, in which the anterograde IFT does not depend on OSM-3. Our results show that this protein is a stoichiometric component of the IFT particle complex B and interacts directly with complex B subunit IFT46. In concurrence with the established IFT protein nomenclature, DYF-1 is also named IFT70 after the apparent size of the protein. IFT70/CrDYF-1 is essential for the function of IFT in building the flagellum because the flagella of IFT70/CrDYF-1–depleted cells were greatly shortened. Together, these results demonstrate that IFT70/CrDYF-1 is a canonical subunit of IFT particle complex B and strongly support the hypothesis that the IFT machinery has species- and tissue-specific variations with functional ramifications.
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小鼠IFT复合物的表征B。
影响因子：
--
作者：
Follit, John A.;Xu, Fenghui;Keady, Brian T.;Pazour, Gregory J.
通讯作者：
Pazour, Gregory J.
DOI：
10.1083/jcb.102.5.1797
发表时间：
1986-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bloodgood RA;Woodward MP;Salomonsky NL
通讯作者：
Salomonsky NL
影响因子：
64.8
作者：
Ou, GS;Blacque, OE;Scholey, JM
通讯作者：
Scholey, JM
影响因子：
7.2
作者：
Molnar, Attila;Bassett, Andrew;Baulcombe, David
通讯作者：
Baulcombe, David
Chlamydomonas Reinhardtii鞭毛中PKD2的功能和动力学。
DOI：
10.1083/jcb.200704069
发表时间：
2007-11-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Huang K;Diener DR;Mitchell A;Pazour GJ;Witman GB;Rosenbaum JL
通讯作者：
Rosenbaum JL