The LIS1-related NUDF protein of Aspergillus nidulans interacts with the coiled-coil domain of the NUDE/RO11 protein.
The LIS1-related NUDF protein of Aspergillus nidulans interacts with the coiled-coil domain of the NUDE/RO11 protein.
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DOI：
10.1083/jcb.150.3.681
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发表时间：
2000-08-07
期刊：
影响因子：
--
通讯作者：
Morris NR
中科院分区：
文献类型：
--
作者：
Efimov VP;Morris NR
丝状真菌构巢曲霉（Aspergillus nidulans）的nudF基因在细胞质动力蛋白/动力肌动蛋白途径中起作用，并且是细胞核分布所必需的。NUDF蛋白是nudF基因的产物，与神经元迁移所需的人类蛋白LIS 1具有42%的序列同一性。LIS 1基因的单倍不足导致人类大脑的畸形，称为无脑畸形。我们筛选了nudF基因突变的多拷贝抑制因子。在筛选中分离的nudE基因的产物NUDE是粗糙脉孢菌的核分布蛋白RO 11的同源物。NUDE蛋白的高度保守的NH 2末端卷曲螺旋结构域在过表达时足以实现蛋白质功能。一个类似的卷曲螺旋结构域存在于几种假定的人类蛋白质和X的有丝分裂磷蛋白43（MP43）中。光滑。NUDF蛋白在酵母双杂交系统中与曲霉NUDE卷曲螺旋相互作用，而人LIS 1与NUDE/RO 11卷曲螺旋的人同源物以及非洲爪蟾MP43卷曲螺旋相互作用。此外，NUDF与表位标记的NUDE共沉淀。NUDF和LIS 1与相同的蛋白质结构域相互作用的事实加强了这两种蛋白质在功能上相关的概念。
The nudF gene of the filamentous fungus Aspergillus nidulans acts in the cytoplasmic dynein/dynactin pathway and is required for distribution of nuclei. NUDF protein, the product of the nudF gene, displays 42% sequence identity with the human protein LIS1 required for neuronal migration. Haploinsufficiency of the LIS1 gene causes a malformation of the human brain known as lissencephaly. We screened for multicopy suppressors of a mutation in the nudF gene. The product of the nudE gene isolated in the screen, NUDE, is a homologue of the nuclear distribution protein RO11 of Neurospora crassa. The highly conserved NH2-terminal coiled-coil domain of the NUDE protein suffices for protein function when overexpressed. A similar coiled-coil domain is present in several putative human proteins and in the mitotic phosphoprotein 43 (MP43) of X. laevis. NUDF protein interacts with the Aspergillus NUDE coiled-coil in a yeast two-hybrid system, while human LIS1 interacts with the human homologue of the NUDE/RO11 coiled-coil and also the Xenopus MP43 coiled-coil. In addition, NUDF coprecipitates with an epitope-tagged NUDE. The fact that NUDF and LIS1 interact with the same protein domain strengthens the notion that these two proteins are functionally related.
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影响因子：
3.6
作者：
Minke, PF;Lee, IH;Plamann, M
通讯作者：
Plamann, M
影响因子：
--
作者：
Morris, SM;Anaya, P;YuLee, LY
通讯作者：
YuLee, LY
影响因子：
64.8
作者：
HATTORI, M;ADACHI, H;INOUE, K
通讯作者：
INOUE, K
DOI：
10.1111/j.1432-1033.1996.0400h.x
发表时间：
1996-09-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Brunati, AM;James, P;Pinna, LA
通讯作者：
Pinna, LA
DOI：
10.1006/bbrc.2000.2243
发表时间：
2000-03-05
影响因子：
3.1
作者：
Hayashi, MAF;Portaro, FCV;de Camargo, ACM
通讯作者：
de Camargo, ACM