S100A8 and S100A9 inhibit neutrophil oxidative metabolism in-vitro: involvement of adenosine metabolites.
S100A8 and S100A9 inhibit neutrophil oxidative metabolism in-vitro: involvement of adenosine metabolites.
复制标题
DOI：
10.3109/10715760903431434
复制
发表时间：
2010-04
影响因子：
3.3
通讯作者：
Marucha PT
中科院分区：
文献类型：
--
作者：
Sroussi HY;Lu Y;Zhang QL;Villines D;Marucha PT
中性粒细胞是天然免疫系统中短命的粒细胞，专门用于产生活性氧物种。S100A8和S100A9及其异源复合体钙保护素在中性粒细胞募集中起作用，占中性粒细胞胞浆蛋白重量的40%。本研究旨在检测S100A8和S100A9对中性粒细胞氧化代谢速率的影响。推测这两种S100蛋白抑制中性粒细胞相关的氧化。对新鲜分离的健康志愿者粒细胞体外氧化二氯荧光素-二乙酸酯(DCFH-DA)的能力进行了检测。结果表明，S100A8和S100A9可抑制中性粒细胞对DCFH-DA探针的自发氧化和刺激氧化。腺苷脱氨酶活性可明显降低S100A8和S100A9对中性粒细胞氧化代谢的抑制作用。P1腺苷受体的抑制剂也降低了S100A8/A9的抗氧化作用，为腺苷代谢产物参与S100A8/A9的抗氧化作用提供了进一步的支持。
Neutrophils are short-lived granulocytic cells of the innate immune system specialized in the production of reactive oxygen species. S100A8 and S100A9 and their heterocomplex calprotectin play a role in neutrophil recruitment and represent 40% of neutrophil cytosolic protein weight. The present study was designed to test the effect of S100A8 and S100A9 on the rate of neutrophil oxidative metabolism. It is hypothesized that the two S100 proteins inhibit neutrophil associated oxidation. Granulocytes freshly isolated from healthy volunteers were tested for their ability to oxidize dichlorofluorescin-diacetate (DCFH-DA) in-vitro. The data showed that S100A8 and S100A9 inhibited spontaneous and stimulated oxidation of the DCFH-DA probe by neutrophils. The inhibition of neutrophil oxidative metabolism by S100A8 and S100A9 was markedly reduced by the enzymatic activity of adenosine deaminase. Inhibitors of the P1 adenosine receptors also reduced the anti-oxidative effect of S100A8/A9 providing further support for the involvement of adenosine metabolites in S100A8/A9 anti-oxidative effect.
登录
查看更多内容
影响因子：
3
作者：
ECKERT, JW;ABRAMSON, SL;BRANDT, ML
通讯作者：
BRANDT, ML
影响因子：
3.1
作者：
Farhadi, A;Keshavarzian, A;Banan, A
通讯作者：
Banan, A
影响因子：
4.6
作者：
Reece, TB;Okonkwo, DO;Kern, JA
通讯作者：
Kern, JA
影响因子：
3.4
作者：
Guerreiro, Jaqueline B.;Porto, Maria Aurélia F.;Carvalho, Edgar M.
通讯作者：
Carvalho, Edgar M.
影响因子：
4.3
作者：
Nakamura, T;Kido, J;Nagata, T
通讯作者：
Nagata, T