The effect of oxyhemoglobin on the proliferation and migration of cultured vascular advential fibroblasts
The effect of oxyhemoglobin on the proliferation and migration of cultured vascular advential fibroblasts
复制标题
氧合血红蛋白对培养血管外膜成纤维细胞增殖和迁移的影响
DOI：
10.1007/978-3-211-75718-5_37
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
陈志
中科院分区：
文献类型：
--
作者：
唐卫华;朱刚;冯华;刘智;JH Zhang;陈志
血管外膜在多种血管疾病状态下被激活，但其在脑血管痉挛中的作用容易被忽视。氧合血红蛋白（oxyHb）是蛛网膜下腔出血（SAH）后脑血管痉挛的重要促痉挛剂之一。本研究采用1-200 μM的oxyHb模拟临床情况，观察了oxyHb对培养的大鼠主动脉平滑肌细胞增殖和迁移的影响。采用MTT法、流式细胞术、单层细胞损伤法和Boyden’s小室迁移法等形态学和生化学技术。结果表明，低浓度oxyHb（1-100 μM）可促进VAF的增殖和迁移。相反，高浓度oxyHb（200 μM）抑制培养的血管外膜成纤维细胞（VAF）的增殖和迁移。本研究为氧合血红蛋白影响VAF的增殖和迁移提供了体外实验证据。本研究结果支持VAF可能在SAH后血管损伤反应中发挥重要作用的假设，并可能成为未来治疗的新靶点。
The vascular adventitia is activated in a variety of vascular disease states but its role in cerebral vasospasm has been easily disregarded. Since oxyhemoglobin (oxyHb) is implicated as one of the most important spasmogens for cerebral vasospasm that follows aneurysmal subarachnoid hemorrhage (SAH), 1–200 μM of oxyHb were used in this study to mimic the clinical situation, and the effects of oxyHb on cells proliferation and migration of cultured rat aortic smooth muscle cells was investigated. Morphological and biochemical techniques, such as MTT assay, flow cytometry cell cycle analysis, monolayer-wounding and Boyden’s chamber migration assay were used. Results showed that low concentration of oxyHb (1–100 μM) increase the proliferation and migration of cultured VAFs. On the contrary, high concentration of oxyHb (200 μM) inhibit proliferation and migration of cultured vascular advential fibroblasts (VAFs). This study providesin vitroevidence that oxyhemoglob in could affect the proliferation and migration of cultured VAFs. The results support the hypothesis that VAFs may play a significant role in the vascular response to injury after SAH, and may be a novel potential therapeutic target in future.
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影响因子：
4.8
作者：
R R Smith-R;B. Clower;G M Grotendorst;N. Yabuno;J. M. Cruse
通讯作者：
R R Smith-R;B. Clower;G M Grotendorst;N. Yabuno;J. M. Cruse
DOI：
10.1165/ajrcmb.22.1.f172
发表时间：
2000
影响因子：
6.4
作者：
Bradley H. Strauss;Marlene Rabinovitch
通讯作者：
Bradley H. Strauss;Marlene Rabinovitch
影响因子：
1.9
作者：
Robert R. Smith;B. Clower;J. Cruse;Yutaka Honma;Joseph L. Haining
通讯作者：
Robert R. Smith;B. Clower;J. Cruse;Yutaka Honma;Joseph L. Haining
影响因子：
1.9
作者：
K. Ogihara;D.H. Barnanke;A. Zubkov;A. Parent;J.H. Zhang
通讯作者：
K. Ogihara;D.H. Barnanke;A. Zubkov;A. Parent;J.H. Zhang
DOI：
10.1152/ajpheart.1999.276.6.h1846
发表时间：
1999-06
期刊：
American journal of physiology. Heart and circulatory physiology
影响因子：
--
作者：
M. Tsutsui;H. Onoue;Y. Iida;Leslie A. Smith;T. O’Brien;Z. Katusic
通讯作者：
M. Tsutsui;H. Onoue;Y. Iida;Leslie A. Smith;T. O’Brien;Z. Katusic