Metal tolerance protein MTP6 is involved in Mn and Co distribution in poplar.
Metal tolerance protein MTP6 is involved in Mn and Co distribution in poplar.
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金属耐受蛋白MTP6参与杨树中Mn和Co的分配。
DOI：
10.1016/j.ecoenv.2021.112868
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发表时间：
2021-10
影响因子：
6.8
通讯作者：
Yinan Yao
中科院分区：
文献类型：
--
作者：
Fengming Yang;Yongfeng Gao;Meng Zhang;Zihao Chen;Jikai Liu;Víctor Resco de Dios;Zhuoxi Peng;Qian Gao;Yinan Yao
随着电动汽车行业需求的蓬勃发展，流入陆地生态系统的锰（Mn）和钴（Co）浓度也大幅增加。虽然这些过渡金属可以促进植物的生长和发育，但在高浓度下可能会变得有毒。因此，了解Mn和Co在植物中的分布，开发新的微生物修复这些重金属污染土壤是非常重要的。本研究筛选到一个编码阳离子扩散促进剂（CDF）蛋白的MTP基因PtrMTP 6，该基因是影响Mn和Co在白杨体内分布的关键基因。PtrMTP 6-GFP融合蛋白与mRFP-VSR 2共定位，表明PtrMTP 6蛋白存在于前液泡区室（PVC）。酵母突变体互补试验进一步证实PtrMTP 6是Mn和Co的转运蛋白，可降低过量Mn或Co对酵母细胞的毒性。组织化学分析表明PtrMTP 6主要在韧皮部表达，表明PtrMTP 6可能参与了Mn和Co在植物韧皮部的转运。在过量钴下，PtrMTP 6过表达白杨品系比对照受到更严重的损害，这是由于幼嫩组织中较高的钴积累。PtrMTP 6过表达线表现出很少的变化，他们的耐受过量锰，虽然年轻的组织也积累了更多的锰。PtrMTP 6在Mn和Co在白杨体内的分布中起着重要作用，深入研究其调控机制对提高Mn和Co污染生态系统的生物修复具有重要意义。
With the booming demand of the electric vehicle industry, the concentration of manganese (Mn) and cobalt (Co) flowing into land ecosystems has also increased significantly. While these transition metals can promote the growth and development of plants, they may become toxic under high concentrations. It is thus important to understand how Mn and Co are distributed in plants to develop novel germplasms for the remediation of these heavy metals in contaminated soils. Here, anMTPgene that encodes the CDF (cation diffusion facilitator) protein inPopulus trichocarpa,PtrMTP6,was screened as the key gene involved in the distribution of both Mn and Co in poplar. The PtrMTP6-GFP fusion protein was co-localized with the mRFP-VSR2, showing that PtrMTP6 proteins are present at the pre-vacuolar compartment (PVC). Yeast mutant complementation assays further identified that PtrMTP6 serves as a Mn and Co transporter, reducing yeast cell toxicity after exposure to excessive Mn or Co. Histochemical analyses showed thatPtrMTP6was mainly expressed in phloem, suggesting that PtrMTP6 probably involved in the Mn and Co transport via phloem in plants. Under excess Co, PtrMTP6 overexpressing poplar lines were more severely damaged than the control due to higher Co accumulations in young tissue. PtrMTP6 overexpressing lines showed little change in their tolerance to excess Mn, although young tissues also accumulated more Mn. PtrMTP6 play important roles in Mn and Co distribution in poplar and further research on its regulation will be important to increase bioremediation in Mn and Co polluted ecosystems.
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影响因子：
14.9
作者：
Dobson L;Reményi I;Tusnády GE
通讯作者：
Tusnády GE
影响因子：
14.9
作者：
Blum M;Chang HY;Chuguransky S;Grego T;Kandasaamy S;Mitchell A;Nuka G;Paysan-Lafosse T;Qureshi M;Raj S;Richardson L;Salazar GA;Williams L;Bork P;Bridge A;Gough J;Haft DH;Letunic I;Marchler-Bauer A;Mi H;Natale DA;Necci M;Orengo CA;Pandurangan AP;Rivoire C;Sigrist CJA;Sillitoe I;Thanki N;Thomas PD;Tosatto SCE;Wu CH;Bateman A;Finn RD
通讯作者：
Finn RD
影响因子：
7.4
作者：
Eroglu, Seckin;Meier, Bastian;Peiter, Edgar
通讯作者：
Peiter, Edgar
影响因子：
2.1
作者：
R. Gopal
通讯作者：
R. Gopal
影响因子：
6.9
作者：
Millaleo R;Reyes-Díaz M;Alberdi M;Ivanov AG;Krol M;Hüner NP
通讯作者：
Hüner NP