Stress-induced metabolic exchanges between complementary bacterial types underly a dynamic mechanism of inter-species stress resistance.
Stress-induced metabolic exchanges between complementary bacterial types underly a dynamic mechanism of inter-species stress resistance.
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DOI：
10.1038/s41467-023-38913-8
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发表时间：
2023-05-31
影响因子：
16.6
通讯作者：
Hwa, Terence
中科院分区：
文献类型：
--
作者：
Amarnath, Kapil;Narla, Avaneesh V.;Pontrelli, Sammy;Dong, Jiajia;Reddan, Jack;Taylor, Brian R.;Caglar, Tolga;Schwartzman, Julia;Sauer, Uwe;Cordero, Otto X.;Hwa, Terence
代谢互食在促进生态多样性方面起着重要作用。虽然一些微生物依赖于交换必需的营养物质来生长，但人们对驱动支持自由生活的微生物共存所需的广泛交叉喂养的力量知之甚少。在这里，我们表征细菌生理学下的自我酸化，并建立广泛的排泄生长停滞后的关键代谢产物提供了一个协作，种间的抗应激机制。这种协作不仅发生在从同一群落分离的物种之间，而且发生在具有互补代谢模式（糖酵解与产气）的不相关物种之间。这些社区的文化进展，通过不同的阶段的生长-稀释周期，包括指数增长，酸化引发的生长停滞，协同脱酸，和生长恢复，每个阶段涉及不同的组合的生理状态的个别物种。我们的研究结果挑战了生态模型中通常描绘的生态系统的稳态观点，提供了一种基于互补物种在不同阶段的生长优势的替代动态观点。微生物可以通过代谢交叉喂养来合作和共享资源。在这里，作者表明，酸应激后关键代谢物的排泄提供了一种协作的、种间的应激抗性机制。
Metabolic cross-feeding plays vital roles in promoting ecological diversity. While some microbes depend on exchanges of essential nutrients for growth, the forces driving the extensive cross-feeding needed to support the coexistence of free-living microbes are poorly understood. Here we characterize bacterial physiology under self-acidification and establish that extensive excretion of key metabolites following growth arrest provides a collaborative, inter-species mechanism of stress resistance. This collaboration occurs not only between species isolated from the same community, but also between unrelated species with complementary (glycolytic vs. gluconeogenic) modes of metabolism. Cultures of such communities progress through distinct phases of growth-dilution cycles, comprising of exponential growth, acidification-triggered growth arrest, collaborative deacidification, and growth recovery, with each phase involving different combinations of physiological states of individual species. Our findings challenge the steady-state view of ecosystems commonly portrayed in ecological models, offering an alternative dynamical view based on growth advantages of complementary species in different phases. Microbes can cooperate and share resources via metabolic cross-feeding. Here, the authors show that excretion of key metabolites following acid stress provides a collaborative, inter-species mechanism of stress resistance.
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