Administration of FGF‐2 to embryonic mouse brain induces hydrocephalic brain morphology and aberrant differentiation of neurons in the postnatal cerebral cortex
Administration of FGF‐2 to embryonic mouse brain induces hydrocephalic brain morphology and aberrant differentiation of neurons in the postnatal cerebral cortex
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将 FGF-2 注射到小鼠胚胎脑中可诱导出生后大脑皮层神经元的脑积水形态和异常分化
DOI：
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发表时间：
2001
影响因子：
4.2
通讯作者：
S. Furukawa
中科院分区：
文献类型：
--
作者：
M. Ohmiya;H. Fukumitsu;A. Nitta;H. Nomoto;Y. Furukawa;S. Furukawa
于胚胎第14天在小鼠脑室注射成纤维细胞生长因子-2，观察其对发育脑形态及大脑皮层多种细胞或分化相关蛋白表达的影响。大剂量的成纤维细胞生长因子-2(200或300 ng)可引起颅骨变形、脑室间隙扩大和大脑皮质变薄等脑部改变。大脑神经元层排列无明显异常，但大脑皮层上层(II/III)和下层(IV-VI)的细胞数量和密度均增加。脑源性神经营养因子(BDNF)、酪氨酸羟化酶、巢蛋白和微管相关蛋白2在大脑皮层深部异常或异位表达。其中有相当数量的细胞共表达这些抗原。这些观察表明，在胚胎14岁时单次注射成纤维细胞生长因子-2后，皮质深层的神经元亚群异常分化或部分维持其未成熟状态。对BDNF阳性细胞定位的发育分析表明，异常始于P5左右。此外，细胞迁移不受成纤维细胞生长因子-2的影响。在发育中的小鼠大脑皮层中，即使在脑神经发生的相对较晚阶段，成纤维细胞生长因子-2似乎在神经元前体的增殖和神经元分化中起主导作用。J.神经学结果65：228-235,2001。©2001 Wiley-Liss公司
Fibroblast growth factor‐2 (FGF‐2) was injected into mouse cerebral ventricles at embryonic day (E) 14 in utero and its effects on developing brain morphology and expression of various cell‐ or differentiation‐associated protein markers in the cerebral cortex were examined. High doses of FGF‐2 (200 or 300 ng) caused encephalic alternations such as deformation of the calvarium, enlargement of the ventricular spaces, and thinning of the cerebral cortex. There was no gross abnormality in the alignment of the cerebral neuronal layers, however, both cell number and cell density of the upper layers (II/III) and the lower layers (IV–VI) of the cerebral cortex were increased. Brain‐derived neurotrophic factor (BDNF), tyrosine hydroxylase, nestin, and microtubule‐associated protein 2 were aberrantly or ectopically expressed in the deep areas of the cerebral cortex. A substantial number of these cells coexpressed these antigens. These observations demonstrate that a subpopulation of neurons in the cortical deep layer abnormally differentiated or partly sustained their immature state following a single administration of FGF‐2 at E14. Developmental analysis of localization of BDNF‐positive cells suggested that the abnormality started around P5. Furthermore, cell migration was not affected by FGF‐2 administration. FGF‐2 seems to play predominant roles in the proliferation of neuronal precursors and in neuronal differentiation in the developing mouse cerebral cortex even at relatively late stages of brain neurogenesis. J. Neurosci. Res. 65:228–235, 2001. © 2001 Wiley‐Liss, Inc.
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DOI：
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发表时间：
1992-03
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
T. Tohyama;V. Lee;L. Rorke;M. Marvin;R. McKay;J. Trojanowski
通讯作者：
T. Tohyama;V. Lee;L. Rorke;M. Marvin;R. McKay;J. Trojanowski
DOI：
--
发表时间：
1994
期刊：
Journal of neurobiology
影响因子：
--
作者：
F. Eckenstein
通讯作者：
F. Eckenstein
DOI：
10.1073/pnas.90.8.3602
发表时间：
1993-04-15
影响因子：
11.1
作者：
RAY, J;PETERSON, DA;GAGE, FH
通讯作者：
GAGE, FH
DOI：
10.1073/pnas.89.2.648
发表时间：
1992-01-15
影响因子：
11.1
作者：
LINDVALL, O;ERNFORS, P;PERSSON, H
通讯作者：
PERSSON, H
影响因子：
3.3
作者：
COFFIN, JD;FLORKIEWICZ, RZ;DOETSCHMAN, T
通讯作者：
DOETSCHMAN, T