Regulation on Expression of Toll-like Receptors on Monocytes After Stimulation with the 3-o-C12-HSL Molecule from Pseudomonas aeruginosa
Regulation on Expression of Toll-like Receptors on Monocytes After Stimulation with the 3-o-C12-HSL Molecule from Pseudomonas aeruginosa
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铜绿假单胞菌 3-o-C12-HSL 分子刺激后单核细胞 Toll 样受体表达的调节
DOI：
10.1007/s00284-012-0162-z
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发表时间：
2012-06
影响因子：
2.6
通讯作者：
Zhong, Haiying
中科院分区：
文献类型：
--
作者：
Lu, Qi;Lin, Yujia;Yang, Xiqiang;Liu, Wei;Zhang, Xianhong;Huang, Daochao;Zhong, Haiying
群体感应（Quorum Sensing，QS）是一种细胞间通讯。铜绿假单胞菌QS分子N-3-（氧代十二烷酰基）-L-高丝氨酸内酯（3-o-C12-HSL）具有调节其宿主免疫系统的潜力。然而，这一活动的机制尚待充分说明。为了能够理解这种活性，我们确定了3-o-C12-HSL是否对单核细胞中的免疫功能和toll样受体（TLR）的表达具有直接影响。单核细胞与不同浓度（0、10、25、50和100 μmol/L）的3-o-C12-HSL共同培养12 h后，单核细胞产生IL-12受到抑制，单核细胞增殖受到阻滞，TLR 2-和4-mRNA表达减少，TLR 5-mRNA表达增加，呈剂量依赖性。3-o-C12-HSL在最低浓度（10 μmol/L）时也能显著诱导单核细胞mRNA表达的变化（P < 0.05）。有趣的是，尽管TLR 2和TLR 4蛋白水平降低，但TLR 5蛋白表达没有改变。这些发现为理解铜绿假单胞菌感染中慢性炎症的持续性提供了新的视角。他们还表明，TLR 2，4和5在单核细胞活化过程中可能不共享相同的信号通路。
Quorum sensing (QS) is a type of cell-to-cell communication. The Pseudomonas aeruginosa QS molecule N-3-(oxododecanoyl)-L-homoserine lactone (3-o-C12-HSL) has the potential to modulate the immune system of its host. However, the mechanism of that activity is yet to be fully characterized. To be able to understand this activity, we determined whether 3-o-C12-HSL has a direct effect on the immune function and the expression of toll-like receptors (TLRs) in monocytes. Monocytes were cultured with 3-o-C12-HSL at different concentrations (0, 10, 25, 50, and 100 μmol/L) for 12 h; upon exposure to 3-o-C12-HSL, IL-12 production in monocytes was inhibited, monocyte proliferation was blocked, TLR2- and 4-mRNA expressions were reduced, and TLR5-mRNA expression was increased in a dose-dependent manner. Strikingly, 3-o-C12-HSL was able to significantly induce mRNA changes in the monocytes even at the lowest concentration (10 μmol/L, P < 0.05). Interestingly, though TLR2- and 4-protein levels were reduced, TLR5 protein expression was not changed. These findings provide a new perspective toward understanding the persistence of chronic inflammation in P. aeruginosa infections. They also suggest that TLR2, 4, and 5 may not share the same signaling pathways during monocyte activation.
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DOI：
--
发表时间：
2002-03
期刊：
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
影响因子：
--
作者：
C. Feng;Wen-li Ma;Yan-bing Song;Qiu-ye Guo;Qing-hua Wu;Wenling Zheng
通讯作者：
C. Feng;Wen-li Ma;Yan-bing Song;Qiu-ye Guo;Qing-hua Wu;Wenling Zheng
影响因子：
5.5
作者：
Beutler, B;Hoebe, K;Ulevitch, RJ
通讯作者：
Ulevitch, RJ
影响因子：
4.4
作者：
Skerrett, SJ;Liggitt, HD;Wilson, CB
通讯作者：
Wilson, CB
影响因子：
--
作者：
Hotchkiss, RS;Tinsley, KW;Karl, IE
通讯作者：
Karl, IE
影响因子：
3.1
作者：
T. G. Jahr;L. Ryan;A. Sundan;Henri S. Lichenstein;G. Skjåk-Bræk;T. Espevik
通讯作者：
T. G. Jahr;L. Ryan;A. Sundan;Henri S. Lichenstein;G. Skjåk-Bræk;T. Espevik