Surface sensing in Vibrio parahaemolyticus triggers a programme of gene expression that promotes colonization and virulence.
Surface sensing in Vibrio parahaemolyticus triggers a programme of gene expression that promotes colonization and virulence.
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DOI：
10.1111/j.1365-2958.2010.07445.x
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发表时间：
2011-01
影响因子：
3.6
通讯作者：
McCarter LL
中科院分区：
文献类型：
--
作者：
Gode-Potratz CJ;Kustusch RJ;Breheny PJ;Weiss DS;McCarter LL
副溶血性弧菌通过阻碍其极地鞭毛的旋转来感知表面。我们利用这种表面感知机制来欺骗有机体，让它在液体中生长时认为自己在表面上。这促进了对全球基因表达的研究，避免了许多表面对液体比较的复杂情况，并阐明了对表面感应本身做出反应的约70个基因。几乎所有的基因都是表面诱导的(不是抑制的)，并编码成群的运动蛋白、毒力因子或与化学接收和c-di-GMP信号有关的感觉酶。进行了后续研究，以将表面反应基因置于调控层次中。绘制层级图揭示了关于LafK的两个惊喜，LafK是一种转录激活因子，到目前为止一直被认为是侧鞭毛系统的主要调节因子。首先，LafK控制着一组比之前想象的更多样化的基因。其次，一些LAF基因不在LafK的控制之下，这意味着LafK毕竟不是主调控因子。由转录组分析推动的其他实验表明，在表面生长可以降低c-di-GMP水平并增强细胞毒性。因此，我们证明了副溶血性弧菌在遇到表面时可以调用基因控制程序，并且表面反应基因的特异性与定植和致病有关。
Vibrio parahaemolyticus senses surfaces via impeded rotation of its polar flagellum. We have exploited this surface sensing mechanism to trick the organism into thinking it is on a surface when it is growing in liquid. This facilitated studies of global gene expression in a way that avoided many of the complications of surface-to-liquid comparisons, and illuminated ~70 genes that respond to surface sensing per se. Almost all are surface-induced (not repressed) and encode swarming motility proteins, virulence factors, or sensory enzymes involved with chemoreception and c-di-GMP signaling. Follow-up studies were done to place the surface-responsive genes in a regulatory hierarchy. Mapping the hierarchy revealed two surprises about LafK, a transcriptional activator that until now has been considered to be the master regulator for the lateral flagellar system. First, LafK controls a more diverse set of genes than previously appreciated. Second, some laf genes are not under LafK control, which means LafK is not the master regulator after all. Additional experiments motivated by the transcriptome analyses revealed that growth on a surface lowers c-di-GMP levels and enhances cytotoxicity. Thus, we demonstrate that V. parahaemolyticus can invoke a program of gene control upon encountering a surface and the specific identities of the surface-responsive genes are pertinent to colonization and pathogenesis.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
5.1
作者：
Lai, Sandra;Tremblay, Julien;Deziel, Eric
通讯作者：
Deziel, Eric
影响因子：
3.2
作者：
Kim, YK;McCarter, LL
通讯作者：
McCarter, LL
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3.2
作者：
Enos-Berlage, JL;McCarter, LL
通讯作者：
McCarter, LL