Physiological and pharmacological basis of GABA and glycine action on neurons of mudpuppy retina. III. Amacrine-mediated inhibitory influences on ganglion cell receptive-field organization: a model.
Physiological and pharmacological basis of GABA and glycine action on neurons of mudpuppy retina. III. Amacrine-mediated inhibitory influences on ganglion cell receptive-field organization: a model.
复制标题
GABA 和甘氨酸对泥犬视网膜神经元作用的生理和药理学基础。
DOI：
10.1152/jn.1981.45.4.783
复制
发表时间：
1981
影响因子：
2.5
通讯作者：
Dacheux,RF
中科院分区：
文献类型：
--
作者：
Frumkes,TE;Miller,RF;Slaughter,M;Dacheux,RF
抑制性氨基酸γ-氨基丁酸（GABA）和甘氨酸（Gly）似乎被用作脊椎动物视网膜中的神经递质。放射性标记摄取和释放研究（33，35）、放射自显影（17-19，36，54）、生化分析（32，33）和合成酶的免疫细胞化学定位（68）表明这些药物具有神经分泌作用（53，64）。在以前的论文（46-48）中，我们通过神经药理学实验为GABA和Gly的递质作用提供了进一步的证据，这些实验表明，外源性应用的GABA/Gly在双极、无长突和神经节细胞部位具有与内源性抑制性递质影响相似的作用机制。在无长突细胞和神经节细胞水平，我们证明GABA/Gly作用是由氯离子电导增加介导的;由于神经节细胞水平的大多数（如果不是全部）IPSP也是氯离子依赖性的（44），外源性GABA/Gly和内源性抑制作用之间类似的离子基础作用至少在定性上是确定的。GABA/Gly拮抗剂的应用也提供了对GABA/Gly可能被内源性利用的特定位置的了解。这些研究结果表明，在mudpuppy视网膜中，GABA和甘氨酸能通路主要位于内层视网膜中（46-48）。此外，在双极水平存在GABA与Gly作用的分离，其中开启双极更敏感，而关闭双极相对更敏感（47）。光诱发的GABA/Gly作用似乎叠加在这些（或类似）试剂的连续黑暗释放上（47，48）。牛磺酸是一种基于神经药理学实验的可行的候选递质，在所有视网膜水平上具有与Gly相似的作用（7，8）。尽管两栖动物的水平细胞似乎摄取标记的GABA（64），但我们无法提供任何证据证明泥狗中GABA介导的水平细胞作用（47）。人们普遍认为，水平细胞是双极动物的拮抗性环绕反应的基础（26，43，49- 51，65）。在泥小狗中，双极的拮抗性环绕反应未被阻断
The inhibitory amino acids y-aminobutyric acid (GABA) and glycine (Gly) appear to be utilized as neurotransmitters in the vertebrate retina. Radiolabeling uptake and release studies (33, 35), autoradiography (17-19, 36, 54) biochemical analysis (32, 3 3) and immunocytochemical localization of synthesizing enzymes (68) point to a neurosecretory role for these agents (53, 64). In previous papers (46-48) we have provided further evidence for a transmitter role for GABA and Gly by neuropharmacological experiments, which show that exogenously applied GABA/Gly have a similar mechanism of action to that of endogenous inhibitory transmitter influences at bipolar, amacrine, and ganglion cell sites. At the amacrine and ganglion cell level, we demonstrated that GABA/Gly action is mediated by an increase in chloride conductance; since most, if not all, of the IPSPs at the ganglion cell level are also chloride dependent (44), a similar ionic basis of action between exogenous GABA/Gly and intrinsic inhibitory action is at least qualitatively established. The application of GABA/Gly antagonists have also provided insight into specific locations at which GABA/Gly may be endogenously utilized. These findings suggest that in the mudpuppy retina, GABA-and glycinergic pathways are predominantly in the inner retina (46-48). In addition, there is segregation of GABA versus Gly action at the bipolar level where on bipolars are more GABA sensitive, whereas off bipolars are comparatively more Gly sensitive (47). Light-evoked GABA/Gly actions appear to be superimposed on continuous dark release of these (or similar) agents (47, 48). Taurine, a viable transmitter candidate based on neuropharmacological experiments, has an action similar to that of Gly at all retinal levels (7, 8).Although amphibian horizontal cells appear to take up labeled GABA (64), we were unable to provide any evidence for GABA-mediated horizontal cell action in the mudpuppy (47). It is widely believed that horizontal cells underlie the antagonistic surround response of bipolars (26, 43, 49-5 1, 65). In the mudpuppy the antagonistic surround response of bipolars was not blocked
登录
查看更多内容
某些不饱和类固醇与酸性铁试剂的反应
影响因子：
7.4
作者：
B. Zak;N. Moss;A. Boyle;A. Zlatkis
通讯作者：
A. Zlatkis
角黄素制剂中的顺式角黄素和其他类胡萝卜素化合物
DOI：
--
发表时间：
1988
期刊：
影响因子：
--
作者：
S. Mayne;R. Parker
通讯作者：
R. Parker
消化道渗出素质
影响因子：
64.8
作者：
H. Dam;J. Glavind
通讯作者：
J. Glavind
影响因子：
4.4
作者：
G. Combs
通讯作者：
G. Combs
DOI：
--
发表时间：
1986
期刊：
影响因子：
--
作者：
J. Colford;R. Parker
通讯作者：
R. Parker