Circulating microRNAs as potential biomarkers of aerobic exercise capacity.
Circulating microRNAs as potential biomarkers of aerobic exercise capacity.
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DOI：
10.1152/ajpheart.00711.2013
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发表时间：
2014-02-15
期刊：
影响因子：
--
通讯作者：
Thum T
中科院分区：
文献类型：
--
作者：
Mooren FC;Viereck J;Krüger K;Thum T
microRNAs （miRs）是多种生物过程的重要细胞内介质，也影响心血管系统。最近，研究表明，miRs在细胞外的血液中循环，这种循环的miRs随着身体活动而变化。因此，本研究的目的是研究马拉松跑前、跑后和跑后24小时个体血浆中心脏/肌肉特异性和炎症相关的mir，并分析它们与常规生化、心血管和性能指标的关系。在进行一系列心功能测试后，招募了14名男性耐力运动员（n =14）进行研究。在公开马拉松比赛之前、之后和24小时后采集血液样本。使用单个Taqman法测量miR-1、miR-133、miR-206、miR-499、miR-208b、miR-21和miR-155，并归一化为秀丽隐杆线虫miR-39（细胞-39）峰值对照。此外，测定了可溶性心脏、炎症和肌肉损伤标志物。结果，骨骼和心脏肌肉的特异性miRs在马拉松后显著增加。miR-206的增加最为明显。跑步24小时后，只有miR-499和miR-208b恢复到运动前水平，而其他的仍然增强。相比之下，miR-21和-155不受运动的影响。miR-1、-133a和-206与有氧性能参数相关，如最大摄氧量（V * o2max）和个人厌氧乳酸阈值（VIAS）下的跑步速度。miR-1与分数缩短呈中度负相关，而miR-133a与脑室间隔厚度呈正相关。mir均与心肌损伤标志物如肌钙蛋白T、肌钙蛋白I和前脑利钠肽无关。总之，这些发现表明肌肉和心脏特异性miRs在耐力运动后心血管适应过程中的潜在作用。此外，miR-1、-133a和-206与性能参数的特定相关性表明，它们可能是有氧能力的生物标志物。
Purpose microRNAs (miRs) are crucial intracellular mediators of various biological processes, also affecting the cardiovascular system. Recently, it has been shown that miRs circulate extracellularly in the bloodstream and that such circulating miRs change in response to physical activity. Therefore, the purpose of the current study was to investigate heart/muscle specific and inflammation related miRs in plasma of individuals before, directly after, and 24 h after a marathon run and to analyze their relation to conventional biochemical, cardiovascular, and performance indexes. Male endurance athletes (n =14) were recruited for the study after performing a battery of cardiac functional tests. Blood samples were collected before, directly after, and 24 h after a public marathon run. miR-1, miR-133, miR-206, miR-499, miR-208b, miR-21, and miR-155 were measured using individual Taqman assays and normalized to Caenorhabditis elegans miR-39 (cel-39) spike-in control. Moreover, soluble cardiac, inflammatory, and muscle damage markers were determined. As a result, skeletal- and heart muscle-specific miRs showed a significant increase after the marathon. The strongest increase was observed for miR-206. Twenty-four hours after the run, only miR-499 and miR-208b were returned to preexercise levels, whereas the others were still enhanced. In contrast, miR-21 and -155 were not affected by exercise. miR-1, -133a, and -206 correlated to aerobic performance parameters such as maximum oxygen uptake (V̇o2max) and running speed at individual anaerobic lactate threshold (VIAS). miR-1 showed a moderate negative correlation with fractional shortening, whereas miR-133a was positively related to the thickness of intraventricular septum. None of the miRs correlated with cardiac injury markers such as troponin T, troponin I, and pro-brain natriuretic peptide. In conclusion, these findings suggest a potential role for muscle- and heart-specific miRs in cardiovascular adaptation processes after endurance exercise. Moreover, the specific correlation of miR-1, -133a, and -206 to performance parameters indicated their potential role as biomarkers of aerobic capacity.
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影响因子：
3.7
作者：
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