High-throughput chemical and chemoenzymatic approaches to saccharide-coated magnetic nanoparticles for MRI.
High-throughput chemical and chemoenzymatic approaches to saccharide-coated magnetic nanoparticles for MRI.
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DOI：
10.1039/c9na00376b
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发表时间：
2019-09-11
影响因子：
4.7
通讯作者：
Webb, Simon J.
中科院分区：
文献类型：
--
作者：
Fallows, Thomas W.;McGrath, Andrew J.;Silva, Joana;McAdams, Simon G.;Marchesi, Andrea;Tuna, Floriana;Flitsch, Sabine L.;Tilley, Richard D.;Webb, Simon J.
许多生物医学应用需要生物功能化磁性纳米颗粒，包括具有一系列表面特性和官能团的 MRI 造影剂。使用高通量化学合成方法创建了用于纳米颗粒功能化的十一种加合物库，每种加合物都是通过还原糖与儿茶酚酰肼缩合而形成的。通过 β-1,4-半乳糖基转移酶将 N-乙酰氨基葡萄糖 (GlcNAc) 加合物酶促转化为 N-乙酰基乳糖胺加合物，这说明了化学酶方法如何提供带有复杂且昂贵的聚糖的加合物。超顺磁性氧化铁纳米颗粒（直径 8 nm，通过 TEM、DLS 和 SQUID 表征）涂有这些加合物，并测定了 GlcNAc 标记纳米颗粒的磁共振成像 (MRI) 特性。这种简单的方法可以生产一系列具有各种生物功能化表面的 MRI 造影剂。涂有聚糖-儿茶酚加合物的磁性纳米粒子是从通过简单缩合反应和酶催化反应创建的库中选择的，作为 MRI 造影剂进行评估。
There is a need for biofunctionalised magnetic nanoparticles for many biomedical applications, including MRI contrast agents that have a range of surface properties and functional groups. A library of eleven adducts, each formed by condensing a reducing sugar with a catechol hydrazide, for nanoparticle functionalisation has been created using a high-throughput chemical synthesis methodology. The enzymatic transformation of an N-acetylglucosamine (GlcNAc) adduct into an N-acetyllactosamine adduct by β-1,4-galactosyltransferase illustrates how chemoenzymatic methods could provide adducts bearing complex and expensive glycans. Superparamagnetic iron oxide nanoparticles (8 nm diameter, characterised by TEM, DLS and SQUID) were coated with these adducts and the magnetic resonance imaging (MRI) properties of GlcNAc-labelled nanoparticles were determined. This straightforward approach can produce a range of MRI contrast agents with a variety of biofunctionalised surfaces. Magnetic nanoparticles coated with a glycan-catechol adduct, selected from a library created through a simple condensation reaction and an enzyme-catalysed reaction, were assessed as MRI contrast agents.
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影响因子：
17.4
作者：
Gustafson HH;Holt-Casper D;Grainger DW;Ghandehari H
通讯作者：
Ghandehari H
影响因子：
2.2
作者：
GUERON, M
通讯作者：
GUERON, M
影响因子：
4.3
作者：
Baudendistel, Oliver R.;Wieland, Daniel E.;Wittmann, Valentin
通讯作者：
Wittmann, Valentin
影响因子：
8.4
作者：
Basuki, Johan S.;Esser, Lars;Davis, Thomas P.
通讯作者：
Davis, Thomas P.
影响因子：
0.8
作者：
Fallows, Thomas W.;Coxon, Thomas P.;Webb, Simon J.
通讯作者：
Webb, Simon J.