Insoluble Fiber in Barley Leaf Attenuates Hyperuricemic Nephropathy by Modulating Gut Microbiota and Short-Chain Fatty Acids.
Insoluble Fiber in Barley Leaf Attenuates Hyperuricemic Nephropathy by Modulating Gut Microbiota and Short-Chain Fatty Acids.
复制标题
DOI：
10.3390/foods11213482
复制
发表时间：
2022-11-02
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
高尿酸血症（HUA）以血清尿酸（UA）水平异常为特征，被认为是高尿酸血症肾病（HN）的重要危险因素，与肠道微生物群密切相关。研究了大麦叶片不溶性纤维（BL）对腺嘌呤（Ad）和氧酸钾（PO）诱导的HN的保护作用和调控机制。结果显示，BL可显著降低血清UA和肌酐（CR）水平，减轻肾损伤和纤维化。此外，BL可调节HN小鼠肾脏氧化应激，下调尿酸转运蛋白1 （URAT1）和葡萄糖转运蛋白9 （GLUT9）的表达。此外，16S rRNA序列数据显示，BL还增加了产生短链脂肪酸（SCFAs）的细菌的相对丰度，包括拟杆菌（Bacteroides）、Alloprevotella和Eisenbergiella。此外，BL处理也使SCFAs水平升高。有趣的是，在高尿酸血症小鼠中应用SCFAs可有效降低其血清UA。此外，SCFAs在体外剂量依赖性地抑制URAT1和GLUT9，并在对接分析中与URAT1和GLUT9相互作用。综上所述，我们的研究结果表明，BL及其代谢物SCFAs可能是缓解HUA或HN的潜在候选者。
Hyperuricemia (HUA), characterized by abnormal serum uric acid (UA) levels, is recognized as an important risk factor for hyperuricemic nephropathy (HN), which is strongly linked to gut microbiota. This study investigated the protective effects and regulatory mechanisms of insoluble fiber from barley leaves (BL) against HN, induced by adenine (Ad) and potassium oxonate (PO). The results showed that BL dramatically reduced the levels of serum UA and creatinine (CR) and alleviated renal injury and fibrosis. Moreover, BL modulated oxidative stress and downregulated the expression of urate transporter 1 (URAT1) and glucose transporter 9 (GLUT9) in the kidneys of mice with HN. In addition, the 16S rRNA sequence data showed that BL also increased the relative abundance of short-chain fatty acids (SCFAs)-producing bacteria, including Bacteroides, Alloprevotella, and Eisenbergiella. Besides, BL treatment also increased SCFAs levels. Of interest, the application of SCFAs in hyperuricemic mice effectively reduced their serum UA. Furthermore, SCFAs dose-dependently inhibited URAT1 and GLUT9 in vitro and potently interacted with URAT1 and GLUT9 in the docking analysis. When taken together, our results indicate that BL and its metabolite SCFAs may be potential candidates for relieving HUA or HN.
登录
查看更多内容
影响因子：
4.6
作者：
Guo Z;Zhang J;Wang Z;Ang KY;Huang S;Hou Q;Su X;Qiao J;Zheng Y;Wang L;Koh E;Danliang H;Xu J;Lee YK;Zhang H
通讯作者：
Zhang H
影响因子：
5.9
作者：
Li D;Feng Y;Tian M;Hu X;Zheng R;Chen F
通讯作者：
Chen F
影响因子：
5.9
作者：
Camerotto, Carla;Cupisti, Adamasco;Gallieni, Maurizio
通讯作者：
Gallieni, Maurizio
DOI：
10.1053/j.ajkd.2017.12.009
发表时间：
2018-06
期刊：
American journal of kidney diseases : the official journal of the National Kidney Foundation
影响因子：
--
作者：
Johnson RJ;Bakris GL;Borghi C;Chonchol MB;Feldman D;Lanaspa MA;Merriman TR;Moe OW;Mount DB;Sanchez Lozada LG;Stahl E;Weiner DE;Chertow GM
通讯作者：
Chertow GM
DOI：
10.1159/000484271
发表时间：
2018-01-01
期刊：
URIC ACID IN CHRONIC KIDNEY DISEASE
影响因子：
--
作者：
Andrade Sierra, Jorge;Flores Fonseca, Milagros M.
通讯作者：
Flores Fonseca, Milagros M.